Effects Of Benzo (a)pyrene Of A Coking Plant On The Air Pollution And On Learning And Memory In Rats

PART I COKING PLANT JIULONGPO AREA WITH BENZO(A)PYRENE POLLUTION SURVEYObjective: To use high performance liquid chromatography analysis of Jiulongpo Area with the air of coking plants benzo (a) pyrene pollution, of benzo (a) pyrene pollution on human health.Methods:①The oven at the top of coke plant, coke oven side, oven bottom and the control area (a university campus) to set three sampling points, using the dust samplers to sample, using High Performance Liquid Chromatography coking operating environment benzo (a) pyrene concentrations;②From the coking plant at 1000, 2000, 3000, 4000 m locale 3 samples, using the dust samplers to sample, High Performance Liquid Chromatography determination of coking plant from the area surrounding the air in different benzo (a) pyrene concentration;③In the coking plant were chosen around a business district, traffic areas and living areas as the sampling area, using the dust samplers to sample, High Performance Liquid Chromatography comparative analysis of different functional areas around the coking plant in the air in different quarters of benzo (a) pyrene concentration differences.Results: (1) Coke oven air in the oven top and oven side and oven bottom of benzo (a) pyrene was significantly exceed occupational health standards (0.15μg / m3), the control area did not exceed the standard. It shows that benzo (a) pyrene concentration from oven bottom to the top of a significant increase in coke oven. (2)From the coking plant closer regional benzo (a) pyrene, the higher the concentration, the difference was statistically significant (P<0.05). (3)Coke plant benzo(a)pyrene concentration in the first quarter higher, the lower the concentration of the third quarter. Coking plant one, two, three, four quarters of benzo (a) pyrene levels were significantly higher than the surrounding functional areas.There was significant difference (P <0.05).Conclusion: The production of coke plant emissions are caused by benzo (a) pyrene pollution of the major sources of pollution, not only for the region and the surrounding environment of the poor air quality impact, and may be affect the surrounding population health risk factors.PARTⅡEFFECTS OF BENZ(A)PYRENE ON CAPABILITY OF LEARNING AND MEMEORY IN RATSSectionⅠBenzo (a) pyrene induced spatial memory behavior change Objective: Chronic intraperitoneal injection of benzo (a) pyrene to establish animal models of different doses of benzo (a) pyrene for learning and memory ability of rats.Methods: Male SD rats were weaned in early 40, were randomly divided into 4 groups: solvent control group, benzo (a) pyrene in the high dose group (6.25mg/kg body weight), medium dose group (2.5mg/kg body weight) and low-dose group (1mg/kg body weight). 10 each, 2 times per week (Monday, Friday) intraperitoneal injection time for consecutive 12 weeks (24 times). After the poisoning, with Morris water maze learning and memory tests to determine different doses of benzo (a) pyrene on spatial cognition, memory effect.Results: (1) flight test scheduled, with the exposure dose increased in rats exposed to various groups, the average latency to find platform, the last number of cross-platform significantly reduced compared with the control group. Each dose group and control group were significantly different (P<0.05), and high dose group compared with the low dose group was significantly different between (P<0.05). (2), dose group, the average percentage of search for effective strategies to increase with dose reduction, the differences were significant (P <0.05).(3) in the space search trial, the dose of the 1st group, the latency to find the original with increasing dose was prolonged, the number of the last collapse and in the former platform quadrant time are associated with exposure dose not decrease, the difference was significant (P <0.05).Conclusions: (1) benzo (a) pyrene in learning and memory of rats exposed to a certain extent by the role; (2) different doses of benzo(a)pyrene on rat learning and memory damage in a dose-response relationship .Section II Benzo (a) pyrene-induced morphological changes in hippocampalObjective: To observe the benzo (a) pyrene induced changes in hippocampal morphology, in order to further confirm that benzo (a) pyrene and learning and memory in rats is closely correlated.Methods: Rats grouping and treatment as above Section. Behavior after the end of the experiment by intraperitoneal injection of pentobarbital anesthetized rats, with 4% paraformaldehyde for infusion into brain tissue perfusion craniotomy remove fluid, using HE staining, light microscope morphological changes in the hippocampus; use 2.5% glutaraldehyde perfusion, obtained and isolated rat brain hippocampus.Results: (1) light microscopy and the control group exposed to normal hippocampal morphology, nerve cells and nerve fibers were arranged regularly, the nucleus is clearly visible; (2) the observation group the normal ultrastructure of hippocampal neurons , 1mg/kg some exposure in hippocampal neurons concentrated degeneration, nuclear shrinkage distortion; 2.5mg/kg exposure group showed mitochondrial swelling; 6.25mg/kg Golgi appears expanded exposure group phenomenon.to observe the ultrastructural changes in hippocampus.Conclusion: light and electron microscopy showed a certain degree of hippocampal morphological changes in structure that benzo (a) pyrene of chronic exposure on ultrastructure of hippocampal neurons damaging effect, while the hippocampus is an important functional areas of learning and memory, and learning and memory are closely linked to infer benzo (a) pyrene chronic exposure to a certain extent, affect learning and memory in rats.