The Separation And Determination Of Ketoprofen In Plasma By Chiral Column

The separation effect for the enantiomers of ketoprofen on four diferent polysaccharide-derivative chiral stationary phases was investigated and a stereospecific HPLC method was developed for the determination of ketoprofen enantiomers in animal plasma. The enantiomers of ketoprofen were resolved in the normal phase mode by using N-hexane- isopropanol-TFA( 80:20:0.02,v/v/v)as mobile phase at the flow rate of 1.0ml/min with the UV detector at a wavelength of 254 nm. The resolution is more than 2.0, the detection limit (S/N=3 ) was 2 ng for each enantiomer. Linear calibration curves were obtained in the range 0.1–10μg/ml for R-(+)-ketoprofen (r=0.9998) and S-(+ )-ketoprofen (r=0.9996) in plasma.This method is simple, fast and can be used to detect optical purity of S-(+)-ketoprofen and monitor pharmacokinetic studies of ketoprofen enantiomers.