| Background and objective: Interleukin-17A belongs to a family of six members (IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F) and has been of great interest recently owing to the discovery that the production of IL17-A characterizes a subset of CD4+ helper T cells (Th17 cells). The development of Th17 cells is distinct from the evelopment of Th1, Th2 and regulatory T cells and is characterized by unique transcription factors and cytokine requirements. Elevated IL-17 levels were found in RA, SLE, psoriasis patients, though it was not clear from these studies how important a role it might play in disease pathogenesis. Therefore, it is meaningful to prepare and identify monoclonal antibody (mAb) against human Interleukin-17A.Methods: BALB/c mice were immunized with hIL17-A, and hybridoma cell lines were obtained by fusing mouse spleen cells with myeloma NS-1 cells. The specificity of mAbs were characterized by enzyme-linked immunosorbent assay (ELISA), Western blot, immuno- histochemical staining.Results: 7 hybridoma cells which secreted the mAbs to hIL17-A were obtained. Six of them were identified as the IgM isotype, and the one of them was identified as the IgG2a isotype. Western blot analysis showed that the mAbs could recognize a target molecule with relative molecular mass (Mr) of 15400. Immunohistochemical staining revealed that the reactivities of 7 mAbs to the epithelial cells in stomach cancer tissues and esophagus cancer were positive.Conclusion: The mAb preparations in the present study will be raised valuable tools for studies on inflammatory and cancerousdiseases.
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