An Initial Explorer For CXCR7/SDF-1 Effect On Endothelial Progenitor Cells

Endothelial progenitor cells (endothelial progenitor cells, EPCs) from the bone marrow release into the peripheral blood, disease damage region involved in angiogenesis treatment of angiogenesis-related diseases, an important way.Mobilization of EPCs from the bone marrow, migration, homing to sites of angiogenesis, the process involved in angiogenesis by exogenous signals and internal factors of the cascade dynamic regulation. Stromal cell-derived factor -1 (Stromal cell-derived factor-1, SDF-1) is an important signal of cells exogenous chemokine binding to its receptor can regulate many important biological reactions. CXCR7 (chemokine (CXC motif) receptor 7) is a recently discovered the new SDF-1 receptor, but SDF-1 / CXCR7 is involved in regulation of angiogenesis-related home and abroad, little reported in the literature. EPCs as a focal point of this study, investigated in vitro SDF-1 / CXCR7 on progenitor cell proliferation, migration, adhesion and apoptosis and to explore regulation of CXCR7 EPCs in SDF-1 mobilization, homing role during the initial explore the mechanism in order to demonstrate to CXCR7 as targets for promoting or inhibiting angiogenesis, the possibility to as angiogenesis related diseases, diagnosis and treatment of new theory. The main research contents and results as follows:â‘ By density gradient centrifugation of bone marrow mononuclear cells by EBM-2 medium for showing "Star Colony" and the typical structure, specific markers of endothelial progenitor cells by CD31 and the VE-cadherin, and Ac-LDL and UEA-1 identification to prove that bone marrow-derived mononuclear cells can be induced to become functional EPCs.â‘¡The expression of CXCR4 and CXCR7 on EPCs cells was detected with Reverse transcription-PCR and western-blot. Proliferation of the EPCs cells was detected by MTT, after they were blocked by anti-CXCR4,anti-CXCR7 respectively. The effect of anti-CXCR4 and anti-CXCR7 respectively on the Chemotaxis of the Hepatoma cells were detected by Transwell chamber Chemotaxis experiments. By the treatment of the anti-CXCR4,anti-CXCR7,to explore the adhesion of EPCs cells on the HUVEC.The concentrations of CXCR4 and CXCR7 were expressed on EPCs cells. Anti -CXCR7 abolished significantly the effects induced by CXCR7 on Proliferation. The CXCR7 was no significant role in the migration of EPCs cells . The both of anti-CXCR4 and anti-CXCR7 impacted on the adhesion of EPCs cells in vitro, However ,the anti-CXCR7 was more pronounced inhibitory effect than the anti-CXCR4. It is concluded that the SDF-1 plays a direct promoting role in proliferation, migration, and adhesion of the Hepatoma cells .CXCR7 plays a different but synergistic role as compared with that of CXCR4.Anti-CXCR7 possibly abolished the induction, It is concluded that the SDF-1 plays a direct promoting role in proliferation, migration, and adhesion of the Hepatoma cells .CXCR7 plays a different but synergistic role as compared with that of CXCR4. Anti-CXCR7 possibly abolished the induction, so that it is potential as a therapy target for cardiovascular diseases.