The Application Of Chromosome Analyzing In Diagnosis And Treatment With CMPD

Objective:To investigate the clinical significance of chromosome analyzing in classification, diagnosis and efficacy to determine with the myeloproliferative disorders (CMPD).Methods:Making a collection of bone marrow cells with CMPD were prepared by short-time culture, karyo-typing was performed by R-banding, dynamic detection of chromosomal changes were carried out in some patients in the course of progress.Results:â‘ The detection rate of chromosomal abnormality in 159 cases of CMPD patients was 69.18%, of which, in 122 cases of CML patients, was 85.25%(104/122), in 37 cases with newly diagnosed other types CMPD patients, the detection rate was 16.22% (6/37).â‘¡The rate of extra chromosome was found in 4.42% of patients in chronic phase (CP), and 61.11% in accelerated phase (AP) and blast crisis phase (BP), which had significant difference(X2=15.228, P<0.01).â‘¢Ph chromosome negative rate in CML patients with Gleevec therapy compared with bone marrow transplantation after treatment was not significantly different (X2=0.0178, P>0.05).Conclusions:There is a close correlation between complex chromosome abnormalities and the progress of CML. Chromosome analysis plays an important role in the classification, diagnosis, and efficacy to determine of CMPD.