The Expression Of Bcl-2,PCNA In Endostatin Gene Therapy On Endometriosis In Nude Mice

Objective:To determine the effects of endostatin on experimental endometriosis in nude mice, this study uses nude mice as experimental models for endometriosis, and measures the expression of Bcl-2 and proliferating cell nuclear antigen(PCNA) in ectopic endometrum of nude mice.Methods:Six cases who had myoma of uterus and accepted the operation of complete uterectomy in the department of the Second Hospital of Tianjin Medical University were selected, mean age is 45 years old. Postoperative pathology verifies that secretes the membrane in an uterus. Sixty female nude mice were choosed,and adopt abdominal cavity plant law to set up uterus membrane malposition nude mice's model of disease. Sixty nude mice with endometriosis were divided randomly into three groups:the endostatin group(n=20),the group without endostatin(n=20),the PBS group(n=20).The method of administration is as follows:during the second laparotomy the endostatin group inject the adenovirus with the endostatin 1*109v.g./100μl in the ectopic foci;the group without endostatin inject the adenovirus without the endostatin 1*109v.g./100μl in the ectopic foci; the PBS group inject the PBS 1*109v.g./100μl in the ectopic foci. Laparotomies were performed to each group two weeks after the treatment. At the same time we put the nude mice of every group to death, left the focus of fetching, observed the change of the body figure of gland in ectopic foci of every group.Using SP immunohistochemical technique,we assessed Bcl-2 and PCNA in ectopic endometrium of model mice.Results:(1)The model of endometriosis in nude mice was established successfully with an achievement ratio of 100%. (2) Before the medication,the gland numbers in the three groups were not significantly different(P>0.05). After the medication 2 weeks, under the light microscope,the gland in three groups withered,the blood vessel in three group reduced. The endostatin group gland reduced obviously, and the mirror displays in the group without endostatin was similar to the PBS group.(3)The expression of Bcl-2 protein is mainly in the glandular epithelium,and less in the cell nucleus,little in the internal membrane blood vessel. The expression of PCNA protein is mainly in the cell nucleus, less in the cytoplasma.(4)Bcl-2 and PCNA were both located in the gland through curing. The positive rate of expression about Bcl-2 protein in the endostatin group after the medication was significantly lower than that in the endostatin group before the medication (P<0.05); The positive rate of expression about Bcl-2 protein in the group without endostatin and the PBS group after the medication was not significantly different from that of before the medication(P>0.05); After the medication,the positive rate of expression about Bcl-2 protein in the endostatin group was significantly lower than that in the group without endostatin(P<0.05) and in the PBS group (P<0.05); After the medication,the positive rate of expression about Bcl-2 protein in the group without endostatin was not significantly different from that of the PBS group(P>0.05). The positive rate of expression about PCNA protein in the endostatin group after the medication was significantly lower than that in the endostatin group before the medication (P<0.05); The positive rate of expression about PCNA protein in the group without endostatin and the PBS group after the medication was not significantly different from that of before the medication(P>0.05); After the medication,the positive rate of expression about PCNA protein in the endostatin group was significantly lower than that in the group without endostatin(P<0.05) and in the PBS group (P<0.05); After the medication,the positive rate of expression about PCNA protein in the group without endostatin was not significantly different from that of the PBS group(P>0.05). Conclusions:The method of establishing the model of endometriosis in nude mice is reliable, and with a high achievement ratio. The species is near human and the results of the experiment are reliable. After the treatment by endostatin the volume of ectopic endometriotic lesions were significantly reduced,the gland were withered,the blood vessel were reduced, and the expression of Bcl-2,PCNA were decreased. Therefore we can conclude that the endostatin can directly inhibit the proliferation of endometriotic cells, inhibit the angiogenesis of ectopic lesions, and thereby inhibit the growth of ectopic endometriotic lesions. Endostatin is likely to become a new therapy for endometriosis.