Prescription Of Cooling Blood And Removing Stasis: The Research On The Hot As Inflammation-related Index In The Treatment For The Acute Apoplexy For The Haemostasis And Heat Syndrome

Research purposesThe "stagnated-heat" is difficult for a variety of medical treatment of one of the main causes.In recent years, the "stagnated-heat" theory has been listed as National Key Basic Research Development Program-973 projects and conducted clinical research system.It is put forward by ZhouZhong-ying that the bloods stasis and heat accumulated inside the brain is the key pathogenesis for acute Cerebral hemorrhage stroke. The main treatment is to be given with the Prescription of Cooling Blood and Removing Stasis,including mang functions like clearing away heat,cooling the blood and stopping bleeding,promoting blood circulationg to remove blood stasis,clearing organs and purging heat,etc. The issue of the "stagnated-heat" theory treating medical treatment is difficult and the law of the nature of the molecular basis of syndrome research to patients with acute hemorrhagic stroke study, the use of theoretical, clinical, laboratory research methods combined to reveal the "stagnated-heat" cause of acute hemorrhagic stroke in the pathogenic mechanism and the "stagnated-heat" nature.Thus expand the doctrine of the stagnated-heat treatment in a medical treatment in the application difficult. Indicators of patient blood samples from clinical testing to evaluate the Liangxuetongyu of cerebral hemorrhage in patients with acute period the exact effect of the wind.Research Methods1.Expresses the spectrum chip screening cerebral hemorrhage stroke acute stage before the person entire genome team "hematoma" patient and must "none-hematoma" patient treats with the thermal imagery inflammation responded that the related gene the difference expression as well as cerebral hemorrhage stroke acute stage "the hematoma" the patient treats before and after the thermal imagery inflammation response related gene difference expression.2.Application of ELISA detection of acute intracerebral hemorrhage stroke of "hematoma" patient and "none-hematoma" patient of serum IL-13,IL-1,NF-κB, HSP-70, HSP27 The content and cerebral hemorrhage stroke patients before and after the treatment serum IL-1,NF-κB, HSP-70 of the content.3.Using MTT method and cell supernatant NO content detection to study the prescription of Cooling Blood and Removing Stasis concentration on the blood vessels of different parties endothelial inflammatory injury model by the cell culture in vitro to bacterial endotoxin(LPS) caused by human vein endothelial cells(HUVECs) injury model.4. Cell cycle by flow cytometry to study the detection of different concentrations Liangxuetongyu intervention drugs on LPS-induced of VECs'cell cycle.5.Observating the ultrastructure by transmission electron microscopy to study Liangxuetongyu with different concentrations on LPS-induced cell damage in the state to improve the role of VECs.The results1. Before the cerebral hemorrhage stroke acute stage stasis hot patient treated, compared with after the non-stasis hot patient, the treatment stasis hot patient altogether screens 34 to have the change with the inflammation factor related gene signal passage, with IL-2, HSP, IL-lr, IL-12, IL-3,TGE-β,Fas and TNF-a, IL-4, IL-27, IL-6, the IL-23 related gene signal passage's expression had the remarkable difference; Just before the cerebral hemorrhage stroke acute stage stasis hot patient treats, and the non-stasis hot patient treats compares, has the upward with the TNF related 10 genes to be more than twice of expression differences, has the upward with the HSP related 6 genes to be more than twice of expression differences, the tendency is obvious; The cerebral hemorrhage stroke acute stage stasis hot patient passes the stasis side treatment after the cold hearted compares before the treatment, has the decline with the TNF related 10 genes to be more than twice of expression differences, has the decline with the hot shock protein HSP related 3 genes to be more than twice of expression differences, the tendency is obvious. In prompt cerebral hemorrhage stroke acute stage "the hematoma" has the inflammation pathology's existence relation with the "heat", the cold hearted possibly passes the stasis side the curative effect which responded regarding cerebral hemorrhage stroke acute stage thermal imagery inflammation also with these related gene signal passage's expression change to concern. TNF, HSP are participate in cerebral hemorrhage stroke acute stage thermal imagery inflammation response the important medium, in cerebral hemorrhage damages in the process to play importantly lies between leads the role, blocks the harm which TNF causes possibly to become protects the important direction which stroke cerebral hemorrhage's is worth studying, the HSP high expression may take one kind to the stroke cerebral hemorrhage acute stage stasis hot card diagnosis and the prognosis judgment has the use value norm for reference.2.Cerebral hemorrhage stroke in patients with acute blood stasis heat treatment of serum IL-1, NF-κB, HSP-70, HSP27 levels in patients than in none-heat stasis significant upward trend; cerebral hemorrhage in acute stroke patients before treatment of serum Stagnant Heat in IL-13 levels in patients than in non-heat stasis is also an upward trend,indicating a "hot as" the existence of pathological inflammation,and secretion of endogenous inflammatory factors increase may be due to stasis in patients with self-activated to resist heat Antagonism of inflammatory mediators damage mechanism;cerebral hemorrhage stroke patients with combined western side Liangxuetongyu after treatment compared with before treatment, serum NF-κB,HSP-70 levels decline significantly; which HSP-70 levels in Western medicine in the treatment group and western medicine combined treatment group between the two groups had significant differences; other IL-1 levels are also declining.Tips acute intracerebral hemorrhage stroke period, "Stasis resistance qiao certificate" "hot as" nature is associated with inflammatory pathology; Liangxuetongyu prescription to inhibit the cerebral hemorrhage stroke patients in acute inflammation-related factors and signaling pathway, For further reveal the acute phase of stroke, cerebral hemorrhage,"hematoma heat" type of "heat" may the nature and Liangxuetongyu Recipes mechanism of acute intracerebral hemorrhage has played an important role.3.After the stimulation by LPS for 24h,36h, HUVECs on the decline in MTT reduction capacity, meddle and large doses of the drug group and model group were significantly different, indicating that the Prescription of Cooling Blood and Removing Stasis unreasonable period of time for different concentrations of inflammation in injury of vascular endothelial cells have significant protective effects. Prior to the Prescription of Cooling Blood and Removing Stasis given after LPS induced cell injury models, NO release in cell supernatant significantly reduced compared with the model group were significantly different, cells were significantly reduced damage,indicating the Prescription of Cooling Blood and Removing Stasis side to antagonize vascular endothelial cells from LPS induced inflammatory injury.4. Detected by flow cytometry, to 24h after LPS stimulation model group compared with the control group, S phase cells was increased,G2-M phase cells decreased,while cell debris falling rate than the control group increased significantly, DNA fragments of the histogram peak can be seen clearly that VECs serious injury, cell state is poor. Compared with model group, given the high concentration of drug group Liangxuetongyu side after 24h,S phase cells have a downward trend,G2-M phase increase in the number of cells,DNA fragmentation rate in the histogram shows cells The downward trend in both the model group, the fragment peak also reduced, indicating that Liangxuetongyu side in the above-mentioned time period,the high concentration of drug group inhibited VECs injury, can improve the status of injured cells.5.LPS model was seen under transmission electron microscope observation of cell ultrastructure injury group significantly abnormal,small,medium and large doses of three drugs VEC only mild injury, most cells and normal cells was similar,especially in large doses the state's most organelles close to normal cells,indicating that the different concentrations of Prescription of Cooling Blood and Removing Stasis have the significant improvement about the VECs on LPS induced cell damage.