| Diabetic Nephropathy is one of Diabetes Mellitus's common chronic micro vascular complication of diabetes,and it is also the leading cause of death. In the developed countries,the end stage renal disease which is caused by Diabetes has become the leading cause of kidney failure. China also has this trend. Therefore, it will be of great significance to explore the pathogenesis of diabetic nephropathy and seek effective new drugs for prevention of diabetic nephropathy.PurposeTo investigate the invasion and the mechanism of renal safety Granules on Diabetic Nephropathy and to provide experimental evidence for the clinical treatment of diabetic nephropathy by using STZ (Streptozotocin, STZ) to induce mouse model of diabetic nephropathy and give renal safety Granules.Method54 mice were randomly divided into control group and model group, the 45 mice in the model group were injected with intraperitoneal of STZ pulse(STZ-150mg·kg-1) to result in C57BL/6 mouse model of diabetic nephropathy then they were randomly divided into model group, irbesartan group, renal safety of high, medium and low dose group. After 4 weeks,we tested the relative kidney weight,blood, urine biochemistry, renal function, renal pathological changes,RT-PCR, and immunohistochemistry and observed the mice with diabetic nephropathy and renal CTGF mRNA expression.ResultsBlood glucose:compared with the normal group, the model group mice's blood glucose and 24 hour urinary protein were significantly increased (P<0.01).24-hour urine protein:compared with the normal control group, the model group was significantly higher (P<0.01); compared with model group, each drug was significantly lower (P<0.05,P<0.01);compared with the irbesartan group, the renal safety of three doses particles were not significantly different (P> 0.05);the renal safety of high dose group was lower than the grain, low-dose group, but the difference was not statistically significant.Weight and relative kidney weight in mice:compared with the normal control group, the model group's weight decreased significantly (P<0.01),relative kidney weight increased significantly (P<0.01);compared with the model group, the body weight the drug intervention groups was significantly higher (P<0.05),relative kidney weight decreased significantly (P<0.01);compared with irbesartan group, there was no significant difference of the relative kidney weight and body weight in renal safety of three dose groups (P> 0.05); renal safety of high dose group than in particles,the low dose group decreased, but the difference was not statistically significant.Blood biochemistry:compared with the normal control group, model group mice BUN, Scr was significantly higher (P<0.01); compared with the model group each drug group BUN, Scr was significantly lower (P<0.05,P<0.01),Renal safety of three dose groups compared with the irbesartan group was no significant difference (P>0.05);compared with the grain, low-dose group, renal safety of high dose group decreased, but the difference was not statistically significant.Renal pathology:the glomerular and tubular structure of the normal group was normal;capillary was clear,showing network, and no proliferation in mesangial area. Compared with model group, glomerular hypertrophy and fibrosis were obvious. Irbesartan group, glomerular renal safety of high dose group, normal renal tubular structure, the internal space there,but irregular, with slight balloon shape. Renal safety of dose group, low dose group of renal glomerular hypertrophy security, capillary network structure collapsed balloon dilatation.Connective tissue growth factor:with the normal group than in the mice renal CTGF mRNA and CTGF protein in cortex was significantly higher (P<0.01);with model group,the intervention groups CTGF mRNA and CTGF protein significantly decreased (P<0.01);renal safety of high dose group and particle effects irbesartan group equivalent security compared to particles in the kidney,lower expression of low-dose group, but the difference was not statistically significant (P>0.05)ConclusionsRenal safety pellet can reduce particle relative kidney weight of diabetic nephropathy in mice, it also can reduce 24-hour urinary protein and decrease serum creatinine and renal pathology of renal disease.It has a definite protective effect, which may work through inhibition of CTGF mRNA and protein in renal cortex inhibit the expression of renal fibrosis,diabetic nephropathy in mice to achieve the purpose of renal injury.
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