Gleditsia Sinensis Extract On Liver Cancer Cell TGF-β₁ Signal Transduction Pathway

Objective: According to recent study found that: transforming growth factor-β(TGF-β) as a multifunctional growth factor, and liver cancer and othertumor development and are closely related. TGF-βsignaling pathway is abnormal liver cancer invasion and metastasis development and an important factor. Chinese medicine in the treatment of liver cancer has its own characteristics, which traditional Chinese medicine as a treatment for tumors of Gleditsia often one of the commonly used drugs, recent research confirmed that the Chinese Gleditsia extract concentrate with anti-cancer properties, can inhibit a variety of human solid tumors, including liver cancer cell growth inhibition. This study, we investigated extract on liver cancer cells Gleditsia TGF-β1 signaling system regulating effects of anti-tumor therapy in clinical medicine to provide experimental basis.Methods: Kunming mice of clean grade were obtained 60, in addition to 10 control mice, the remaining 50 mice were correspondingly transplanted hepatoma cell line H22, the establishment of the right upper limb subcutaneous mouse tumor model, tumor hfter the 50 mice were randomly divided into 5 groups fed treatments were: Gleditsia extract group (high dose group: 18.02g / times; middie dose group: 9.01g / times; low dose group: 4.505g / times ), Jinlong Capsule group (positive control group: 9.01g), saline group (model group: 9.01ml) above 5 groups all give three times a day, and the other 10 control group (normal feeding, not any treatment), After 15 days of continuous oral administration, in the first 16 days all mice were sacrificed, and to take samples (the liver, eyes, blood, etc.), to liver cell biochemistry [liver function, a-L-fucosidase (AFU)], and Molecular Biology (TGF-β1 expression) and so on detection.Results: According to the results of analysis of experimental data, after the administration, Gleditsia extract high, medium and low groups Jinlong Capsule group mice and TGF-β1 expression in mice was significantly higher than model group and control group; Gleditsia extract group AFU and liver function tests in mice decreased with the model index for statistical analysis and comparison of mice, there was significant (P<0.05).Conclusion: Gleditsia extract on liver cancer cells TGF-β1 signaling system to regulate, can enhance the expression of TGF-β1 to inhibit the proliferation of hepatoma ceils and promote apoptosis of hepatoma cells.