| PART 1:Objective: To explore Acantbepanax senticosus saponin(ASS) whether the persistent high intraocular pressure caused by retinal nerve cell layer(RGCL) injury protection.Method: Healthy SD rats were 60, including 6 normal control group, 6 normal treatment group (daily ASS200mg/kg), the remaining 38 mining burning branding law, branding right eye closed three rats superficial veins of the sclera, to choose the intraocular pressure stabilized at test requirements(intraocular pressure>26mmHg) of 30 rats were randomly divided into normal saline group and treatment group, saline group 2ml normal saline administered once per day treatment group, the concentration by ASS into A,B,C,D group, concentrations were 50,100,150,200mg/kg,n=6. Treatment time for the month of the experiment rats were killed after the expiry of the removal of the eye, making conventional paraffin, HE staining of retinal thickness, in which the internal limiting membrane of retinal ganglion cell layer to the outside world(X1), within the bundle-like layer and inner nuclear(X2), outer plexiform layer and the the pigment epithelium outside world(X3) was tested from the three sections.Results:1,A normal control group, experimental treatment group with the normal eye (right eye) retinal internal limiting membrane to the ganglion cell layer of the external (X1) thickness count was no significant difference (p>0.05). The normal control group and saline group, the concentration of ASS experimental treatment group compared eye X1 section thickness counts were significantly different (p<0.05), the concentration of ASS experimental treatment group with its own eye-control eye (left eye) X1 section thickness of counts there was a significant difference (p<0.05), saline group and D group of experimental eyes X1 section thickness counts have more significant difference (p<0.05).2.saline group and the experimental concentrations ASS eyes to the inner plexiform layer of the inner nuclear layer (X2) and the outer plexiform layer of the outside world to the retinal pigment epithelium (X3) there is a gradual thinning of the trend, but compared with the control eyes and normal There was no significant difference between eyes (P>0.05). The control eye and the thickness of normal eyes on each floor there was no significant difference (P>0.05).Conclusion:1. ASS of normal rats used alone had no effect on retinal.2.A certain concentration of ASS can cause persistent high intraocular pressure in rat retinal ganglion cell layer provide some protective effect of the damage.Part 2:Objective: To investigate ciwujianoside (Acantbepanax senticosus saponin, ASS) of persistent high intraocular pressure caused by rat retinal ganglion cells (RGC) activity whether there is protection.Methods: SD rats were 20, 6 for the normal control group, normal feeding. I burn branding method used to produce sustained high intraocular pressure in rat models of stability to choose the intraocular pressure in the experimental requirements (intraocular pressure>26 mmHg) in 12 rats were randomly divided into model group (A group) and ASS treatment group (B group). A group of normal saline gavage once a day each 2ml, B Group I ASS150mg/kg to gavage treatment, rats were killed one month after removal of the eye, making eye specimens, routine paraffin sections, AgNOR staining of nuclei of silver staining RGCs particles; TUNEL assay RGCs apoptosis.Results:1. A, B two groups of rats with retinal ganglion cell nucleus silver-staining granules significantly reduced compared with normal group, among the 22 difference was significant (P<0.05).2. Retinal ganglion cell layer in the number of TUNEL-positive cells from high to low followed by A, B group, normal group. The difference among the three groups have two significant (P<0.05).Conclusion:1. Using burning branding law, branding a closed right eye episcleral veins in rats to produce rats with persistent high intraocular pressure model may lead to silver-staining granules within the nucleus RGCs decreased ganglion cell apoptosis.2.ASS on rat ocular injury sustained activity of RGCs and some protective effect.
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