| Objective: To establish experimental autoimmune myasthenia gravis (expermental autoimmune myasthenia gravis, EAMG) rat model, by observing the health drink on the strength of clinical symptoms of EAMG rats, neuromuscular junction (Neuromusculai junction) ultrastructural and CD4~+ cells in peripheral blood lymphocytes change explore the strength Kang drink treatment of myasthenia gravis in rats.Methods: 60 female Lewis rats were randomly divided into blank group, model group, positive medicine group, muscle strength and Sport drink high-dose group, muscle health drink in the dose group, muscle strength and Sport drink low-dose group, n = 10 only. Except the blank group, the remaining rats with AchRα1 129-145 peptide antigen latex modeling. After the success of recognition model, strength Kang drink high, medium and low-dose group, respectively 40g / (kg ? d), 20g / (kg ? d), 10g / (kg ? d) of the Health and dose gavage treatment, positive medicine the control group to pyridostigmine bromide, 4.05g / (kg ? d), twice a day, blank group, model group was given distilled water, treatment for 28 days.After administration of the clinical symptom score in each group and the rats in each group with the electron microscope neuromuscular junction (NMJ) ultrastructure changes. Muscle tissue obtained with the Motic-BA400 microscope under 400 times magnification in the photography, each group randomly selected six horizons, with the Motic image 3.2 image analysis software for analysis, calculation of the average gray value, the reaction changes in the concentration of CD4 ~+ cells.Results: 1.The immune animals 6 weeks after the model prepared by rats in each group of clinical symptoms score, electron microscopy of rat neuromuscular junction ultrastructure compared with the control group were significantly different (P<0.01), in order to determine successful model for the study of the treatment of MG provides a good experimental animal model.2.4 weeks after treatment, muscle strength and Sport drink each group compared with model group, symptom score, neuromuscular junction (NMJ) ultrastructure of a statistical difference (P < 0.05), In peripheral blood lymphocytes CD4~+ cells decreased (P<0.05),muscle strength and Sport drink high-dose group and the positive medicine group there are statistical differences (P<0.05).Conclusion: 1.This experiment AchR-α1 subunit of 129-145 fragment immune Lewis rats, induced symptoms of EAMG rats in the performance of neuromuscular junction ultrastructure performance of the MG with people similar to the initial model to determine the success of To study the anti-drink muscle health conditions MG mechanism.2. Muscle strength and effective health drink treatment of EAMG. Analysis of their mechanisms of intervention might be: through the neuromuscular junction can promote the production of new axons and increase the number of synaptic vesicles and Reduction of CD4~+ cells in peripheral blood lymphocytes to achieve the treatment of MG role.
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