The Expression Of C-Met And MMP-11 In Gastric Carcinoma And Their Relations To Hepatic Metastasis

Objective To investigate the relationship between high expression of C-Met, MMP-11 and gastric cancer hepatic metastasis, and the connection of each factor. We hope we can do something in the early prediction of gastric cancer hepatic metastasis, intensive care and improve the prognosis. Methods Collected the clinical pathology data of 78 patients who were gastric cancer hepatic metastasis and 78 patients who were not gastric cancer hepatic metastasis at the same time in the first affiliated hospital of xinjiang medical university 2002 to 2008, then analyzed the data retrospectively. Checked the filed paraffin wax specimen and the pathologic diagnosis at least by 2 pathological attending physicians. We detected the C-Met, MMP-11 in gastric cancer specimen using in situ hybridization technique and immunohistochemistry technique in DNA and protein levels. Combining with the clinical pathology data, we progressχ2 test and Logistic regression to analyze the Risk factor of gastric cancer hepatic metastasis. Results The result of single factor analysis of variance to clinical pathology data showed that gastric cancer hepatic metastasis correlated with the TNM stage of gastric cancer, general type, degree of differentiation, depth of infiltration, canalis haemalis infiltration, diameter of tumor and lymph node transfusion. The positive rate of C-Met mRNA,MMP-11 mRNA expressing in gastric cancer were 55.13%,56.41% respectively, the positive rate of their expressing in gastric cancer hepatic metastasis were 70.50%,75.60%, comparing with the non-gastric cancer hepatic metastasis,there were significant deviation (P=0.000) ; Meanwhile The expression of C-Met mRNA,MMP-11 mRNA correlated with canalis haemalis infiltration, lymph node transfusion, TNM stage; Of the total, the expression of MMP-11 mRNA correlated with the infiltration depth of gastric cancer. The positive rate of C-Met protein,MMP-11 protein expressing in gastric cancer were 58.97%, 57.69% respectively, the positive rate of their expressing in gastric cancer hepatic metastasis were 67.90%,71.80% respectively, comparing with the non-gastric cancer hepatic metastasis group, there were significant deviation (P<0.005) ; Meanwhile The expression of C-Met protein,MMP-11 protein correlated with lymph node transfusion, TNM stage; Of the total, the expression of MMP-11 protein correlated with the infiltration depth of gastric cancer. The result of Logistic regression to data of gastric cancer hepatic metastasis showed that the general type(different in its multiple classification P=0.003 OR=7.568; P=0.105 OR=4.312), canalis haemalis infiltration (P=0.024 OR=2.841), C-Met mRNA(P=0.017 OR=3.196), MMP-11 mRNA (P=0.020 OR=3.290) and the MMP-11 protein (P=0.081 OR=2.458) were the most important influential factor of gastric cancer hepatic metastasis. Conclusion The excessive expression of c-Met and MMP-11 correlated with infiltration and transfusion, particular with hepatic metastasis, and they may provide objective proof for the early prediction of gastric cancer hepatic metastasis...