Establishment Of Dual Task Model Of Classical Eyeblink Conditioning In Guinea Pigs And Comparison Of Behavioral Parameters

Objective:Cognitive dysfunction is a central nervous system damage many patients can not return to work or participate in normal social life, and it is an important reason to lead to lower quality of life. With the social development,rehabilitation therapy and the prognosis of cognitive dysfunction are more and more paied attention by neurology and rehabilitation doctors.From the middle of the last century, the classical eyeblink conditioning (EBC) has been widely used in the study of neurobiology mechanism of associative learning, which is one of the largest information sources for the formation and storage of mammal's memory.The study on learning and memory made the people have more understanding of brain damage with cognitive impairment,and have played an important role in the rehabilitation assessment and rehabilitation training of cognitive dysfunction and the mechanism of brain functionSome scholars successfully established auditory recognition of training by using guinea pigs,and used the single oxygen flow of sine wave pure tone paring to stimulate two groups of guinea pigs established trace and delay eyeblink conditioning respectively,but it is not reported that the same animal is established traces and delay eyeblink conditioning respectively.The purpose of this study is to establish a dual task model of trace and delay eyeblink conditioning in the same guinea pigs, observe the changes of different parameters during the process of building of the eyeblink conditioning and analyze the acquisition rule of the two kinds of conditioning, which offers a model basis for the study of the accurate function that prefrontal cortex has in the process of brain cognition.Methods:On the basis of the classical eyeblink conditioning, six English male albino guinea pigs were chosen for the research. Each guinea pig ages 8 to 10 weeks and weighs 350 to 450 grams. By using 1kHz and 3kHz sine wave pure tone paring sheaf oxygen flow of 20kPa to stimulate the left cornea of the guinea pigs to train them to establish the trace and delay eyeblink conditioning, muscle tension transducer to collect the eye blinks of the guinea pigs, and Eyeblink conditioning behavior feature sensor signal analyzing software V2.2 to analyze the data of eyeblink, this research carries out a statistical analysis of the various parameters during the process of the establishment of the trace and delay eyeblink conditioning and finds the feasibility and principle of the trace and delay eyeblink conditioning.Results:After 10 days'paring training, all 6 guinea pigs have established the dual task model of trace and delay eyeblink conditioning. The acquisition rates increased along with the increase of training time, which reached 80% on the tenth day.Compared to trace eyeblink conditioning, it is easier for the guinea pigs to establish delay eyeblink conditioning.Although DEBC shows a higher peak amplitudes,but there is no significant difference between TEBC and DEBC peak amplitudes.There is difference in the rates and peak amplitudes of SR between trace and delay eyeblink conditioning,and shows the SR rates and peak amplitudes of TEBC are higher than the SR rates and peak amplitudes of DEBC,while there is no significant difference in the UR peak amplitudes.Conclusion:We successfully establish the dual task model of trace and delay eyeblink conditioning in guinea pigs.There are some rules for the change of parameters during the process of establishing the trace and delay eyeblink conditioning,which offers a model basis for the study of the accurate function that prefrontal cortex has in the process of brain cognition.