An Orthotopic Ovarian Carcinoma Model With Metastasis In Nude Mice

Objective To establish an orthotopic ovarian cancer model with high metastasis in nude mice.Methods Human ovarian carcinoma cells HO-8910PM, a subline with highly metastatic potential, were inoculated subcutaneously in female nude mice to form solid tumors. The exuberant growing tumor were removed, sliced and introduced into ovaries of other nude mice by microsurgical techniques. The growth and metastasis of the orthotopic lesion were followed, and pathological examinations were performed.Results Ovarian cancers formed in animals with an incidence of 57.14%. Metastatic lesions were detected in the lung, diaphragm, liver, stomach, intestine, pancreas, peritoneum, contralateral ovary and lymph nodes located in the iliac fossa, and occurred in 83.33% animals. Conclusion Transplanting tissues into the ovary of a nude mouse can form an orthotopic cancer model, and the manner of cancer spread was similar to that of human lesions, offering a considerable model to explore ovarian cancers.