RAJI Cells Apoptosis-inducing Effects Of Matrine And Its Mechanism

Objectives:Burkitt,s lymphoma is a highly aggressive malignant of Hematopoietic system,endangering the health of human,s life seriously and multidrug resistance occured after chemotherapy easily,therefore,it is necessary to find out new efficient drug to reduce the incidence of multidrug resistance. Matrine is an active substances extracted from the dried root of sophora flavescentait with a wide range of pharmacologic actions.It can resist infection and allergy,enhance immunity and protect the hepatic function,and etc.Recent studies have shown that matrine can induce apoptosis in a variety of malignant tumor cells,but there's no reference about Fas/FasL involved in inducing Raji cells apoptosis by matrine.Our research invests the role of matrine-induced apoptosis of Human Raji cells by observed drug toxicity and the changes of cell apoptosis,and discussed the mechanism of matrine-induced Raji apoptosis by detected the expression of apoptosis protein.Methods:1.The proliferation inhibition rate of Raji cells was performed by MTT analysis and determined the concentration of drug which induced apoptosis.2.Cell apoptosis rate was detected by Annexinâ…¤-FITC/PI double staining method.3.The P-p38MAPK,Fas,FasL and Caspase-3 protein of Raji cells treated with matrine were evaluated by western blot.Results:1.After Raji cells were cocultured with matrine 0.2-1.6 mg/mL for 24,48,72h in vitro,the cell proliferation was inhibited in time-dependent and dose-dependent manner, its half maximal inhibitory concentration (IC50) of 48h was 1.34 mg/mL.2.When the Raji cells were cultured for 48h by matrine 0.4,0.8,1.6 mg/mL,the total apoptosis rate detected by Annexinâ…¤-FITC/PI double staining increased gradually,and had statistical significance compared with control group (P<0.05 or P<0.01). Prior to joining SB203580 (p38MAPK inhibitor), the total rate of apoptosis was lower than the previous.3.The protein expresssion levels of P-p38MAPK,Fas,Fasl and Caspase3 increased after Raji cells were treated by matrine 0.4,0.8,1.6 mg/mL with a concentration-effect relationship, prior to joining SB203580, the above protein expresssion levels was lower than the previous.Conclusions:1.Matrine could inhibit the Raji cells proliferation in time-dependent and dose-dependent manner.2.Matrine could induce the apoptosis of Raji cells in vitro.3.One of its possible mechanisms of matrine-induced Raji apoptosis was that matrine upregulated the levels of Fas and FasL through P-p38MAPK,and then activated caspase-3.