Expression Of AQP8 In Cultured Normal Humantrophocyte And JAR Cell Line

Objective:To discuss the different expression of AQP8 in cultured normal human trophocyte and JAR cell line,and the relationship between the changed expression of the channel protein and nosogenesis of chorionic carcinoma.Methods:The human cultured normal trophocyte was primarily cultured,purified and identified. JAR cell line was cultured.By immunohistochemistry,immunofluorescence confocal laser and RT-PCR, the expression of AQP8 from protein and mRNA levels was detected in cultured normal human trophocyte and JAR cell line.Results:(1) By immunohistochemistry,the limited expression of AQP8 was observed in cultured normal human trophocyte and JAR cell line, but in the exprimental group,the rate of positive cell of AQP8 in JAR cell line was 93.27%±14.45%,much higher than that in the control group.(2) The expression of AQP8 mRNA was detected in both cultured normal human trophocyte and JAR cell line.The OD value(0.98±0.35) of AQP8 in the experimental group was significantly higher than that(0.32±0.04) in the control group. (3) By immunofluorescence staining confocal laser imaging,it was revealed that,in the same condition and excitation intensity,AQP8 in the JAR choriocarcinoma cell lines showed positive expression of the green,while in normal trophoblast cells,no green light,but when the excitation intensity was strengthened,it may still explore AQP8 in normal human villous trophoblast cell expression.Conclusion:In the choriocarcinoma JAR cells,abnormal overexpression of AQP8 suggests that the water and electrolyte balance inside and outside the choriocarcinoma trophoblast cells maybe abnormal.Combined with morphological evolution of trophoblastic disease,the overexpression of AQP8 may have relationship with the occurrence of choriocarcinoma.