Study On Expressions Of The Fas/Fas Ligand On The Lymphocytes In Peripheral Blood In Women With Recurrent Spontaneous Abortion Before And After Active Immunotherapy

Object: Through investigating the expressions of the Fas,Fas Ligand (FasL) on the lymphocytes in peripheral blood and blocking antibody in women with recurrent spontaneous abortion, approach the role of Fas/FasL in the morbidity of RSA and the changes of the expressions of Fas,FasL and blocking antibody after active immunotherapy.Methods: Prospective clinical study. Setting 12 early pregnant RSA patients, 15 nonpregnant RSA patients as experimental groups, and 15 normal early pregnancy, 15 normal nonpregnancy as control groups.(1)Blood samples were obtained from control groups and RSA patients before and after active immunotherapy, blocking antibodies were detected by enzyme-linked immunosorbent assay(ELISA); (2) The expressions of the Fas,FasL on the lymphocytes in peripheral blood in control groups and RSA patients were determined by flow cytometry before and after active immunotherapy.Results: (1) After one course of active immunotherapy, 33.3% early pregnant RSA patients and 20% nonpregnant RSA patients were positive for blocking antibodies; (2) The expressions of Fas in NK cells and CD8+T cells in nonpregnant RSA patients were significantly higher than that of normal nonpregnancy, while the expressions of FasL in NK cells were significantly lower (P<0.05), the expressions of FasL in CD8+T cells and Fas,FasL in CD4+T cells did not differ between two groups (P>0.05);(3) The expressions of Fas,FasL in NK cells in early pregnant RSA patients were significantly lower than that of normal early pregnancy, and the expressions of Fas in CD8+T cells were higher (P<0.05), the expressions of FasL in CD8+T cells and Fas,FasL in CD4+T cells did not differ between two groups (P>0.05);(4) The expressions of Fas in NK cells and CD8+T cells in RSA patients decreased, and the expressions of FasL in which increased after active immunotherapy (P<0.05), the expressions of Fas,FasL in CD4+T cells did not change after active immunotherapy (P>0.05).Conclusions: (1) The hyperexpressions of Fas in NK cells and CD8+T cells , and the hypoexpressions of FasL in which in peripheral blood is one of the important immunological factors which inducing the occurrence of RSA. (2) Active immunotherapy can regulate the expressions of Fas,FasL in NK cells and CD8+T cells in RSA patients, which contributes to reduce apoptotic cell death, and leads to a successful outcome finally.