Effects Of Different Hypoxic Trainings On T, Cand MDA, SOD In Serum Of Sprague-Dawley Rats

Objective:To observe the diversity of testosterone(T),cortisone(C)and T/C of SD rats which have been trained in different hypoxic, provide a eligible hypoxia training mode for athletes. Methods:120 male Sprague-Dawley rats of 8 weeks, which average weight is 182.8±7.3g, were divided randomlt into 6 large groups:control group(C), regular aerobic exercise group(E),15% oxygen concentration control group(15C),15% oxygen concentration exercise group(15E),11% oxygen concentration control group(11c) and 11% oxygen concentration exercise group(11E)(n=20). Each large group divided in the following groups group 1 and 2 (n=10). Animal in exercise group daily exercised on treadmill for 30min once a day and the speed was 12m/min.15C and 11C were placed in the hypoxic room of 15% and 11% oxygen concentration 2h every day.15E and 11E were placed in the hypoxic room of 15% and 11% oxygen concentration 2h every day, the first 30min was treadmill exercise and placed in the hypoxic room 1.5h after exercise. A total of 7 days continuous training. After 7 days'hypoxic training, derived material from group 1 animal. After 15 days, derived the same material from group 2 animal.And the method of detecting the level of serum T and C is ELISA, the method of detecting the level of MDA is TAB, and the method of detecting the level of SOD archaeus is xanthine oxidase (XO). Results (1)group of 11C has the T level lower than group C, the contrast is significant(P<0.05); group of 11E has the T level obviously lower than group E, the contrast is significant (P<0.01) (2) group of E has the C level higher than group C, the contrast is significant (P<0.05); group of 11E has the C level lower than group E, the contrast is significant (P<0.05); group of 15E has the C level lower than group E, the contrast is significant (P<0.05) (3) group of 11C has the T/C level lower than group C, the contrast is significant(P<0.05.)(4) 7 days after the hypoxic training, v MDA content in each group there was no significant difference (P> 0.05); 15 days after the end of hypoxic training, with the quiet control group,15E2 group of MDA were significantly lower than sedentary control group, difference was significant (P<0.05); MDA content before and after two more,11E2 11E1 group MDA was significantly lower than the group, the difference was significant (P<0.01); 15E2 15E1 group MDA was significantly lower than the group, the difference significant (P<0.05) (5) 7 days hypoxic training,11E1 rats SOD activity was significantly lower than E1 group, the difference was significant (P<0.05); 15 days after the end of hypoxic training, with the quiet control compared,11E2 group SOD activity was significantly lower than the sedentary control group, the difference was significant (P<0.05); SOD activity before and after two more, compared with 11C1,11C2 rats, SOD activity was significantly higher 11C1 Group The difference was significant (P<0.05); compared with E1, E2 serum SOD activity was significantly lower than the E1 group, the difference was significant (P<0.05); compared with 15E1,15E2 group Serum SOD activity was significantly lower than the 15E1 group, the difference was significant (P<0.01). Conclusion (1)11% oxygen concentration under the conditions of T/C significantly decreased, suggesting that catabolism is greater than under anabolic body is not conducive to functional levels and improve exercise capacity, although the movement can compensate to some extent the adverse effects of hypoxia, but not enough to compensate; (2)15% oxygen concentration in the body under the T/C has been a favorable trend, possible compensatory movement of the adverse effects of hypoxia, indicating the process of concentration in the hypoxic training is particularly important the choice of suitable oxygen concentration in the Games of the body to produce positive stimuli, suggesting that oxygen concentration in the 15% or slightly higher than the concentration for further study and training, to find the optimal motion compensation point of view to hypoxia and exercise training have the most significant effect. (3) after 7 days hypoxia training,11% oxygen group and 15% oxygen quiet quiet group of rats the concentration of lipid peroxidation did not change, suggesting that 11% of the oxygen concentration in the exercise group increased free radical production in rats, resulting in lipid peroxidation in vivo elevated levels of cells, the body is not conducive to health; while 15% of the oxygen concentration in the exercise group rats oxidative and antioxidant systems are still in good balance. (4) 15 days after the end of hypoxic training,11% oxygen concentration in a quiet group and 15% oxygen concentration in a quiet group of rats in vivo oxidation and antioxidant system is still in balance; 11% of the oxygen concentration in the exercise group and 15% oxygen concentration in the exercise group Tai rats decreased the level of lipid peroxidation, suggesting that hypoxia caused adverse effects on the body has faded.15% of the oxygen concentration in the environment for the end of hypoxic training, reduce the level of lipid peroxidation, while SOD activity caused by decreased adaptability, can save the state as the function of the body. (5)15 days after the hypoxia training on the impact of the body tends to subside.