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The Experimental Research Of Sijunzi Formula On The Free Radical Change In Serum And Liver Of The Training Rats

Posted on:2009-05-27Degree:MasterType:Thesis
Country:ChinaCandidate:P ShangFull Text:PDF
GTID:2167360245462503Subject:Ethnic Traditional Sports
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Research AimsUnder the academic instruction of herbalist doctor basic,by filling rats which were in training states with sijunzi formula of traditional preparation, to observe the effects of sijunzi formula on resisting fatigue and enhancing exercise ability,By mensurating SOD vigor and GSH-PX vigor about enzyme of antioxidation,and the content of MDA in the offspring of free radical metabolism in both the serum and the liver in rats after different exercising states ,to discuss the effects of sijunzi formula and exercise training on rats economy and on the mechanism of enhancing exercising ability,and to establish a base to develop a compound preparation of chinese traditional medicine,which would delay exercise-induced fatigue,improve exercise ability,differ from incitant but safety and efficiency,expecting to provide scientific theory and experimental grounds for people to use chinese traditional medicine reasonably to resist fatigue,and to instruct trainning.Research methodsselect128 health and male Kunming rats,4 weeks old,18-20g,after a week's adaptabe breeding,the rats were divided into 4 groups randomly.they were contrast group(A),exercise group(B),sijunzi formula group(C),exercise+ sijunzi formula group(D),Rats in group A keep quiet and be filled with distilled water,Rats in group B take a swimming exercise and be filled with distilled water,Rats in group C keep quiet and be filled with sijunzi formula,Rats in group D take a swimming exercise and be filled with sijunzi formula,The experiment lasts 6 weeks,and when finishing the experimentation,rats in each group will be divided into 4 different exercising states, there were states of quiet,fixed time(90min's swimming),exhaustion instantly,and exhaustion recovery 24 hours later,at the end of each exercising states, taking some coagulant blood from eyeball to separate serum, and then disconnecting spondyle of the rats to take liver tissue,purging off blood with ice PBS,bloting up blood with filter paper. serum and livers were stord in -30℃to detect SOD,GSH-PX activities,and MDA content,expressing the results with average±stdev(x士s).Research results(1)state of quiet,compared with group A,there were descending trends of MDA contents in rats'serum and liver of groups B and D ,but not markedly(p﹥0.05);there were remarkably increase of GSH-PX,SOD activities in groups B and D(p﹤0.05).(2)at the end of fixed time(90min's swimming), compared with group A, there were remarkably descending of MDA contents in rats'serum and liver of groups B,C and D (p﹤0.05); there were remarkably increase of SOD activities in rats'serum and liver of groups B,C and D (p﹤0.05); there was remarkably increase of GSH-PX activity in rats'serum of group D (p﹤0.05);there were remarkably increase of GSH-PX activity in rats'liver of groups B,C and D (p﹤0.05).(3)exhaustion instantly, compared with group A, there were remarkably descending of MDA contents and remarkably increase of SOD activities in rats'serum and liver of groups C and D (p﹤0.05),compared with group B,there were remarkably descending of MDA contents and remarkably increase of GSH-PX and SOD activities in group D(p﹤0.05); compared with group A,there was remarkably increase of GSH-PX activity in rats'serum and liver of group D(p﹤0.05),there was remarkably increase of GSH-PX activity in rats'liver of group C(p﹤0.05).(4)exhaustion recovery 24 hours later, compared with group A, there were remarkably descending of MDA contents in rats'serum and liver of groups B,C and D(p﹤0.05),of which group D is much lower than that of group B(p﹤0.05);compared with group A, there were remarkably increase of SOD activities in rats'serum and liver of groups C and D(P﹤0.05),there were remarkably increase of GSH-PX activities in rats'liver of groups B,C and D(p﹤0.05);compared with group B there was also remarkably increase of GSH-PX activity in rats'liver of group D(p﹤0.05).(5)compared with group D, the exhaustion time was remarkably prolonged in groups A,Band C(p﹤0.01); compared with group A,the exhaustion time was also remarkably prolonged in groups Band C(p﹤0.05).Conclusions(1)Sijunzi formula can cooperate with exercise training to prolong swimming time exhaustion of rats,there is a obvious effect of sijunzi formula on increasing exercise ability and resisting fatigue.(2)Sijunzi formula can reduce MDA content in rats'serum and liver,restrain lipid oxygenation,and reduce free radical content efficiently.Sijunzi formula can enhance enzyme activities of SOD,GSH-PX in rats'serum and liver,reminding that,sijunzi formula can enhance antioxidation abilities in different training states.(3)Exercise training can reduce MDA content in rats'serum and liver,restrain lipid peroxide,enhance enzyme activities of SOD,GSH-PX ,and boost up antioxidation abilities.
Keywords/Search Tags:Sijunzi formula, exercise training, free radicals, liver tissue, serum, rat
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