Effects Of Fu Shenshen Powder On Learning And Memory In Mice

Effects and mechanisms of modified Fushen Power on Learning and memory of miceObjective To evaluate the effects of modified Fushen Power on learning and memory of normal mice and mouse model with impaired learning and memory, so as to provide experimental evidence for treating mild cognitive impairment.Methods Adult Kunming male mice were randomly divided into normal group, model group, positive drug group (piracetam group at dose of 0.6 g/kg), high dose, middle dose and low dose of modified Fushen Power groups (high-, mid-, low-dose group) at the dose of 22.4 g/kg,11.2 g/kg and 5.6 g/kg respectively. Ethological examination using Morris water maze, locomotor activity system, step-down test and step-through test, was used to study the effects of modified Fushen Power on mice locomotor activities and disorders of memory acquisition consolidation and retrieval due to chemical substances. And the activity of acetyl cholinesterase (AchE), superoxide dismutase (SOD) and content of malondialdehyde (MDA) in brain were detected by chromatographic colorimetry.Results Compared with normal group, the mice in both lose-dose group and mid-dose group showed significant increasing in exploratory behavior at the first 15 minutes (P<0.01). But no difference in the next 15 minutes; furthermore, low-dose and mid-dose Fushen Powder increased the scores of learning and memory. Compared with control group, low, middl, and high dose of Fushen Powder relieved disorder of memory acquisition (P<0.01), in addition, middle dose relieved disorder of memory retrival (P<0.01) and decereased the activity of AchE (P<0.01), increased the activity of SOD (P<0.01), and the content of MDA (P<0.01).Conclusion1. The modified Fushen Powder has no obvious acute toxicity, with increasing the mice’s exploratory behavior in a novel environment and no obvious influence on their locomotive activity.2. The modified Fushen Powder against the disorders of memory acquisition, retention and retrieval induced by scopolamine, sodium nitrite and ethanol were proved, with increasing the normal mices learning and memory. The mechanism of modified Fushen Power on antagonizing the memory disorders can be related to its effects of decreasing activity of AchE, content of MDA, and increasing activity of SOD in brain tissues.3. The modified Fushen Power has different effects on the antioxidative erzymes of D-galactose senescent model.4. The mid-dose group of modified Fushen Powder is more efficient.