Changes Of Serum Creatine Kinase And HMGB1 And Toll - Like Receptors After Trichosanthin In Rats With

Objective:to study the single taste traditional Chinese medicine radix trichosanthis impact experimental autoimmune myositis of rat serum muscle enzymes(creatine kinase and lactate dehydrogenase, aspertate aminotransferase) and the change of HMGB1, TLR2, TLR4 signaling and further understand its a experimental autoimmune myositis rats after radix trichosanthis intervention, muscle inflammation damage under repair and effect mechanism.The experimental process1, immune and distribution group of ratsForty the SPF SD rat feeding training a week later, the laboratory in advance copy completely random design method, the fed in advance of 40 SD rats randomly divided into four categories, respectively: blank group, model group, hormone group, radix trichosanthis.Each group of ten, respectively.By another kind of experimental animal(rabbit) of the raw material of skeletal muscle as a muscle homogenate.After preparation good muscle homogenate muscle homogenate and complete freund’s adjuvant equal parts, each mix them evenly after emulsification.With ten in blank group method without any intervention, before the rest of the thirty to adjust proportion of good muscle homogenate and complete freund’s adjuvant for immunization.Injection location selection in rat back on both sides, subcutaneous injection, injection quantity in 0.35 ml each time, once a week, and in the seventh day, 14 day, 21 days, 28 days, 35 days for a injection in rats, a total of six injection, complete immunity of rats.2, give medicine samples observation and analysisIn the immune after four weeks, rats after building successful drug intervention.Blank group and model group with normal saline to fill the stomach, and the remaining two groups adopt peculiar to each group to lavage treatment.Four groups of lavage around, total four weeks after says the big mouse weight, grouped data, weight determination after anesthesia, take a sample of blood from the abdominal aorta, muscle and other related organizations, after death.3, sample processing and analysis of samplesThrough Lennon LA grading rules of rats after intervention were evaluated by clinical status, and grade;For the last time lavage after comparison between the four groups of weight;Identification of serum creatine kinase and lactate dehydrogenase, aspertate aminotransferase value;By the enzyme-linked immunosorbent method, HMGB1, TLR2, in the muscle of rats and the value of the TLR4 and identification.Results:1 radix trichosanthis can improve creatine kinase(CK), lactate dehydrogenase(LDH), aspertate aminotransferase(AST) in serum levels, reducing the role of CK, LDH, AST2 radix trichosanthis of HMGB1, TLR2, TLR4 conduction effect, thus reducing HMGB1, TLR2, TLR4 content in muscle.Conclusion:Radix trichosanthis can hinder the HMGB1, TLR2, TLR4 conduction;Reduce the content of serum muscle enzymes in the blood.It can prevent the immune factor is the degree of activation, improve the degree of inflammatory cell infiltration healthy muscle tissue, for skin and muscle groups such as inflammation, leading to skin damage played an effective inhibition.So can effectively improve idiopathic inflammatory myopathy the dangers of, and increase the success rate of treatment...