Effects Of P38 Protein Kinase Inhibitor SB203580 On PI3K Pathway In Ovarian Granulosa Cells Of Polycystic Ovary Syndrome

Objective:To investigate the effects of P38 protein kinase inhibitors SB203580 on PI3 K of ovarian granulosa cell of polycystic ovarian syndrome(PCOS).Methods: Ovarian granulosa cell were collected from PCOS patients undergoing in ivtro fertilization/intracytoplasmic sperm injection- embryo transfer(IVF/ICSI-ET),every examples were inoculated in two culture bottle,with inhibitor of P38 mitogen-activated protein kinase as PCOS+IN group(n=10),the others as PCOS group(n=10).Those ovarian granulosa cell from non-PCOS patients undergoing IVF/ICSI-ET used as control group(N-PCOS group)(n=10).The cultured ovarian granulosa cell of three groups were examined by Western Blotting for p-P38 MAPK,phosphorylation of protein kinase B(p-Akt)/Akt and glucose transporter-4.Result:The protein expression level of p-P38 MAPK in the granulosa cell of PCOS patients were higher than N-PCOS group.The protein expression level of p-Akt/Akt and GLUT-4 in the granulosa cell of PCOS patients were lower than N-PCOS group(P < 0.05).The protein expression level of p-P38 MAPK after SB203580 employment were lower significantly,the protein expression level of p-Akt/Akt and GLUT-4 after SB203580 employment were lower than N-PCOS group,but were more than PCOS group(P<0.05).Conclusion :Activity of P38 MAPK signaling pathway in ovarian granulosa cell of PCOS is higher than non-PCOS.Activity of phosphatidylinositol3-kinase(PI3K)/Akt signaling pathway is lower than non-PCOS.There is a link between P38/MAPK and PI3K/Akt signaling pathway,SB203580 may increase the activity of PI3K/Akt pathway and the expression of GLUT-4 through inhibit P38/MAPK signaling pathway.