Functional Identification Of GmSARK:: IPT Fusion Gene And Transformation Of Soybean

An isopentenyl transferase gene (ipt) from T-DNA was transferred into Artemisia annuaL.via Agrobacrerium tumefaciens. The ipt gene under the control of the senescence-specificSAG promoter from Arabidopsis(PSAG12-IPT) significantly delayed developmental andpostharvest leaf senescence in mature heads of transgenic soybean(Lactuca sativa L.Eyola)homozygous for the transgene. In this paper, the recombinant expression vector was transformedinto Arabidopsis thaliana via Floral-Dip.To verify the functions of the GmSARK-IPT gene inArabidopsis thaliana. The recombinant expression vector was transformed into soybean viatransferring with Agrobacterium tumefaciens. To achieved creating of the new drough resistantsoybean germplasm by using the senescence associated receptor protein kinase gene promoterand isopentenyl transferase gene.The specific research results are as follows:1.The recombinant expression vector was transformed into Arabidopsis thaliana viaFloral—Dip. Twenty eight transgene lines were got from transgenic Arabidopsis thaliana.2. Separation ratio of T2generation transgenic plants was tested, the3:1ratio was in accordancewith Mendel’s law of inheritance. It is proved that Arabidopsis transgenic lines we got before issingle copy line.3.Phenotypic analysis of T3generation transgenic plants was tested, when they were underdifferent stress treatments, salt and PEG.The statistical seed germination rate, lateral root lengthand the status of the growth of plants. Functional identification of GmSARK:: IPT fusion gene.4.Soybean materials named“JiYu72”, the recombinant vetor was transformed soybean viaAgrobacterium-mediated method. Heterologous expressing the IPT gene directed by GmSARKpromoter in transgenic plants was first used in this paper.Results, to abtain twelve transgenelines of T0, nine transgene lines of T1, five transgene lines of T2and2transgene lines of T3.