Molecular Phylogenetic Research Of Siobla (Hymenoptera:Tenthredinoidea:Tenthredinidae)

Siobla Cameron (Hymenoptera, Tenthredinidae, Tenthredininae) is the ninth largest genus in Tenthredinidae.It have been reported110kinds from all of the word and81kinds from china. Study on the Siobla genus has been130years of history, having a relatively mature results in the terms of taxonomic research. However, there are still some problems: To the vast majority of species of the genus, their phylogenetic relationship is clear, but it is more difficult to identify some other of the species; Siobla venusta has a wealth of infraspecific category, maybe exist cryptic species or semi-species.In this study, DNA sequence analysis which is a kind of molecular systematics methods was used to the phylogenetic research of Siobla. In this regard, extracted genomic DNA of31species49samples of Siobla (31species30samples were used to be phylogenetic research of Siobla and5species17samples were used to be phylogenetic research of Siobla venusta group) and3outgroup insects(Conaspidia indistincta, Dimorphopteryx tianmunicus and Corymbas glabrifrons), Choosing mitochondrial protein-coding genes COI and ribosomal DNA second internal transcribed spacer (ITS2) as target gene, PCR successfully gained access to50COI gene sequences and27ITS2rDNA sequences, Using MEGA5.0to align sequence and analysis sequence feature, Using PAUP*4.0beta to build phylogenetic tree of Siobla based on ML method、MP method、NJ method and bayesian inference method and build ML tree, NJ tree and BI tree of Siobla venusta group. Get the following conclusions after these analyses:1. Siobla genera: phylogenetic relationships within S. grahami group is ((S graham+S. mooreana)+(S. trilineata+S. turneri)), consistent with the results of morphological taxonomy research; The phylogenetic relationships of some species of S. formosana G. are mainly determined, specifically,(S. parallela+S. pseudoplesia), in line with the results of the morphology can be divided into the same groups. There are closer relationship between S. parallela and S. acutiserrula of S. acutiscutella G.. S. vulgania, S. chengi and S. kanba in S. sturmii G. stay together in certain. S. reficornis and S. liui have a closer relationship. S. ferox G. did not get together different from morphological studies, that still need to find sufficient evidence to ascertain. S. venusta G. also have not a certain relations, which also reflects on the case of more complex relationship in S. venusta G. S. reticulatia, S. obtusiscutellatia, S. zenadia, S. shennongjiana, S. sinica in S. metallica G. are not able to stay together, in which evolutionary relationships need to be further explored. S. acutiserrula of S. acutiscutella G. and S. acutiserrula of S. formosana G. have close relationships.2. S. venusta group: S. nigrocacudalia within S. venusta group have a anterior evolutionary relationship, follows by (S. venusta (Korea)(S. zhengi+S. curvata)), Evolutionary relationship of the test cannot be determined; The relationship between individuals of S. venusta group are connected with geographical location. We should gain more data information to work out final conclusions; S. venusta (Korea) may have a earlier evolutionary. S. venusta complex has very likely geographical species, such as:S. venusta (Korea) could be geographical species.