The Role Of At1G12200Gene In Glucosinolate Biosynthesis And Its Expression Pattern In Arabidopsis Thaliana

Glucosinolate is a kind of plant secondary metabolites in model plant Arabidopsis thaliana, and is also the important sign of cruciferous plants. Structure of glucosinolate comprises thioglucose, thiohydroximate and side-chain. Glucosinolates divide into aliphatic, indole and aromatic because of different side-chains, they hydrolyze various productions by myrosinase. These productions protect plant from harm of some animals and pathogens, some of them have role to inhibition of cancer cell growth. The most famous is methylsulfinylalkyl glucosinolate, which belongs to aliphatic glucosinolate, by the transformation from methylthioalkylalkyl glucosinolate with oxidixation of FMOGs-OXS family. Several kinds of glucosinolates that come from methylsulfinylalkyl glucosinolate have good application prospects, for example, sulforaphane that is breakdown production of4-methylsulfinylbutyl glucosinolate have a strong activity to restrain cancer, and8-methylsulfinyloctyl glucosinolate can reduce Sclerotinia sclerotiorum. So there is an important significance to study the role of FMOGS-OXS family, which can transform methylsulfanyl glucosinolates into methylsulfinylalkyl glucosinolates.According to analysis the sequence, the data suggest that At1G12200gene and FMOGS-OXS belongs to the same family, so we inffered that the gene might also have the ability to modify side-chain of aliphatic glucosinolate, but there is no the gene function reporting. We named FMOGS-OX6instead of At1G12200gene for convenience.For identification the function of the new gene, we constructed the35S::FMO GS-OX6vector, gained the transgenic plant to over express FMOGS-OX6gene, and identified its homozygous mutant from fmoGS-OX6. We compared the glucosinolate content of wild type, overexpress type and knockout homozygous mutant to indentify that FMOGS-OX6gene whether have an ability to oxidize the side-chain of aliphatic glucosinolates. At the same time, we found2000bp sequence as promoter before the gene to constructed the PFMOGS-OX::GUS vector, and dyed with GUS in different growth stages to observe the expression pattern of FMOGS-OX6gene.The result suggests that, contrast to wild and knockout type, the ratios of4-methylsulfinylbutyl,8-methylsulfinyloctyl glucosinolates and their precursor4-methylthioalkylbutyl,8-methylthioalkyloctyl glucosinolates, so the result indicated that FMOGS-OX6gene has an ability to transform the two kinds of methylthioalkylalkyl glucosinolates to methylsulfinylalkyl glucosinolates. The location of GUS suggests that expression of FMOGS-OX6 gene is very low in vegetation growth, except epidermal hair of new leaves, it mainly appears in the germination stage, blossom stage and aging stage. We got the role of FMOGS-OX6gene and its expression pattern basically.