Bioinformatics Mining Of Has-miR-150and Its Potential Targets Amongst Red Cell Membrane Proteins

Erythroid development is a highly ordered process and occurrence of erythropoietic differentiation comprises with a series of great changes such as alternation of composition and structure of membrane protein. The stability of the red cell membrane protein structure and function is a necessity for mature red blood cells to maintain deformation ability and stability.composition of erythrocyte membrane protein was under regulation from multiple levels and factors and miRNA is one kind of novel non-protein regulator for new gene expression which is endogenous non-coding small RNA molecules.(18to25nucleotide).The paper indicates that5kinds of red membrane skeleton protein coded by six coding gene might be the target of has-miR-150through bioinformatic screen, including SLCA1(band3), EPB41(4.1R), ANK1(ankyrin R), GYPC(GPC), SPTA1(α-spectrin) and SPTB(β-spectrin) whose3’UTR have the predicted targets for miR150.Perform analysis of encipher, physicochemical property, transmemebrane structure, modification of amino acid, and secondary or tertiary structure of erythrocyte membrane protein utilizing a variety of software by which provide bioinformatic data to further determine whether has-miR-150targets these genes.The newest pattern of erythrocyte membrane protein was regarded spectrin tetramer as fundamental skeleton of erythrocyte in which one protein site could combine with4.1R, Band3and GPC and another one could be conjoined with ankyrin R and Band3forming4.1R and Band3complexes. Has-miR-150could also target regulate these protein and demonstrate that it play a essential role in the production of erythrocyte membrane protein.