Cloning And Research On The Drought Resistance Of Mir159

Wheat is planted area and production in China, second only to rice, ranked secondin food crops. The drought caused wheat production one of the major stress factors, droughtevery year due to natural wheat production by about20%. There are already a lot of use oftransgenic technology into a single functional genes to improve plant resistance researchreports, although to a certain extent, can improve drought resistance, but improved marginally,drought effect is not ideal. Now on plant drought resistance research focus has graduallyshifted from the function of individual genes identified drought to drought genes involved inthese metabolic pathways and pathway in Stress regulatory mechanism in the process ofrevelation. In recent years, miRNA as a small molecule RNA (Small RNA) in an importanttaxa, and regulation of gene expression as a new factor has drawn increasing attention.According to current research, a miRNA can be complementary base pairing principle in vivomultiple different metabolic pathways regulate target gene mRNA, thereby achieving themultiple metabolic pathway in effect. Taking into account the tolerance of plants to droughtstress is also affected by multiple genes, or even by the number of metabolic pathwaysregulated. Currently people in the plant miRNA various abiotic stress response has carriedout extensive research, the results showed that: in the outside plant abiotic stress or bioticstress, it will modulate their miRNA expression profiles to answer these stresses, and thesemiRNA expression induced by stress may be directly associated with the plant resistance.Previous research has discoveredthat the accumulation of miR159from wheat is upregulatedunder drought stress. On this basis and in order to find the anti-drought function of miR159further, we clone the precursor molecules of miR159from Arabidopsis thaliana by PCRamplification and construct plant expression vector by linking it with35S promoter, thentransferthe miR159into Arabidopsis thaliana over expression via floral dipping method.Finally, through analyzingthe drought resistance of transgenic plants, wepreliminarilyconclude that miR159owns anti-drought function. The main test followingresults，(1)In wild-type Arabidopsis genomic DNA as template, PCR amplification method,successfully cloned miR159gene, and successfully constructed miR159plant expressionvector part27-mir159.(2)By PCR screening and Northern blotting agricultural identification technology, access to the genetic stability of transgenic Arabidopsis plants including miR159.(3)Relative to wild-type Arabidopsis, transgenic Arabidopsis withmiR159-overexpression have obviously drought resistance, and judge miR159function withdrought.(4)According to the experimental data, initially speculated that miR159willproduce a corresponding response to the high salt stress.