Isolation High Chlorophyll Fluorescence Mutant In Arabidopsis Thaliana And Location The Gene

The light energy is absorbed, transferred and converted by chloroplast in higherplants. The light energy is distributed among photosynthesis, chlorophyll fluorescenceand heat dissipation. In order to further clarify the molecular mechanism ofphotosynthetic energy allocation and the linkages between photosynthesis andchlorophyll metabolism, the high chlorophyll fluorescence mutant were screened andthe gene was located.Arabidopsis thaliana Columbia ecotype was used to build EMS mutant library.The M2Arabidopsis was screened to find out high chlorophyll fluorescencephenotype by using PSI Closed FluorCam FC800-C device. One mutant was namedp246-1. The P246-1was used to cross with Arabidopsis thaliana Landsberg erectaecotype. Map-based method was used to identify mutant. The results were as follows:1. Compared with wild type plant, the mutant has small yellow leaf, lowphotosynthetic electron transport rate, low non photochemical fluorescence quenchingand increased chlorophyll fluorescence.2. The AT4G31390gene of p246-1mutant was changed by map-based method.Sequencing results also show that the mRNA alternative splicing error result thefourth intron, fifth and sixth exon together form a big exon, which prevent the proteinto from normal structure in the p246-1mutant. The AT4G31390gene is a member ofABC1/ADCK/UbiB family. The AT4G31390regulated phosphorylation of tocopherolcyclase that control the production of vitamin E and maintained the stability of thepigment and pigment binding protein in pastoglobule.