Analysis Of Midline Functions In Process Of Salivary Gland Migration During Embryonic Stage Of Drosophila Melanogaster

In nature,development of organism depends on the normal running of signaling and accurate control of regulate networks. Follows development, migration on different levels such as cell migration, collective cells migration and tissue migration function coordinately to work. During stage of tissue or organ migration, transcription factors play pivotal roles, their normal expression and regulation is requied for proper migration. When expression of transcription factors appear abnormal, proper migration might be affected and tissue or organ can not reach right target region.We focus on a transcription factor which named as midline, the homolog of midline in vertebrates is tbx20, which belongs to Tbx1 subfamily and expresses and plays pivotal role in different organs and tissues during development of organism.Previous research shows that midline has conserved binding sites and functions in different species by regulating multiply kinds of genes. Meanwhile, midline also be regulated by other signal pathways such as Tinman, Notch, BMP and Pnr. Defections of organism development and maintain will be induced when the expression of midline appears abnormal. Thus, studying the function of midline has a dual role of medical and direct significance for humans.Salivary gland in embryonic stage of Drosophila can serve as ideal model for studying tissue migration, which has simple structure, big size and comprised of two types of cells, therefore, so it’ s convenient for operation and observation. In our research, we use salivary gland of embryonic stage drosophila as major materials and observe and analysis by in situ hybridization, immune staining and other methods.Based on these results, we suggest that Midline is required for proper migration of salivary gland. Midline function in salivary gland migration by regulating fra and slit, the regulation is direct regulation. Distribution of Midline in CNS dominate in controlling salivary gland migration, meanwhile, we construct a model for Mid controls salivary gland migration. Finally, we identify Midline governs salivary gland migration by regulating downstream axon guidance cues.