The Coupling Model Of Ethanol Metabolism Between Arabidopsis Pollen Tube And Stigma Based On The Computational Systems Biology

Plants pollination and fertilization is the basis of the production of the crop yield formation. To illuminate the regulatory mechanism of plants pollination and fertilization is one of the important and hot issues to reproductive and developmental biology research.(1)Based on the open database of Arabidopsis metabolism pathway, the genome-scale metabolic pathway data of Arabidopsis and the multiple omics data in the process of interaction of Arabidopsis pollen tube and stigma were collected and organized.(2)According to the biological and biochemical reaction equation and the construction methods of metabolic interactions network, the genome-scale enzyme correlation network models of Arabidopsis was constructed.(3)Based on the genome-scale transcriptome data of the interaction process between Arabidopsis pollen tube and stigma, the significant differentially expressed genes and the enzymes which were coded by these differentially expressed genes of the pollen tube and the stigmas in response to pollination were identified.(4)Then the enzymes were mapped to the genome-scale enzyme correlation network model, and two sub-interaction networks were constructed.(5)According to the co-regulation and inter-regulation model of the co-expressed enzymes in the consecutive steps for metabolic routes, we identified significant differentially expressed genes that encoded co-expressed enzymes that catalyze the consecutive steps, thereby constructed a metabolic route for ethanol degradation II and tricarboxylic acid cycle variation of the Arabidopsis pollen tube in response to pollination. Our multi-omics network analysis also show an increasing trend of the expression level of genes that encode the co-expressed enzymes and consecutive steps for glycolysis and the key enzymes that produce high-energy nutrients(ethanol) in Arabidopsis stigmas during pollination.(6)Finally, an ethanol metabolism coupling model between Arabidopsis pollen tube and stigma was proposed.Our results will expand the understanding of the molecular regulation mechanism of pollination, as well as could provide research methods to reveal the regulatory mechanism of plants pollination from the perspective of genetics, molecular biology and systems biology.