The Screening Of Antarctic Lignocellulosic Enzyme Production Strains And The Research Of Transcriptome

The climate of Antarctica is cold, gale, strong radiation, low nutrition, where stored the extremely precious rich microbial resources. This paper studied the type and quantity of cuLturable microorganisms based on the ten samples which were collected from Fildes PeninsuLa of Antarctica in the Chinese 30th Antarctic scientific expedition, we alsoanalysed the microbial basic features,distribution and diversity of the area. We screened the bacteria which couLd produce both the low temperature celluLase and ligninase from the Antarctic samples, tested the activity and degradation characteristics, and selected one bacteria which has a high lignocelluLosic enzyme activity, performed transcriptome sequencing and functional annotation analysis.We selected twenty bacterias and five fungus from the cuLtured strains which were collected from the samples at different places of Fildes PeninsuLa, to do some research in morphology, physiological and biochemical, molecuLar identification. The resuLts showed that the twenty bacterias belong to four doors, six classes, nine genera, including 12 bacterias belonging to Proteobacteria, γ-Proteobacteria, and blasted which showed that these bacterias belong to the three genera, Arthrobacter, Pseudomonas,Flavobacterium, then selected two of the four strains of higher lignin enzyme activity and measured celluLase activity, Among them Pseudomonas sp.E4-1 showed a highest ligninase activity, which is chosen to transcriptome analysis. We screened the bacteria produced both the low temperature celluLase and ligninase at the same time from the Antarctic environment, which is rarely reported so far. This couLdlay a foundation for the further study of enzyme production optimization, enzymatic activity properties, functional gene cloning.Pseudomonas sp.E4-l transcriptome were sequenced andassembled by Illumina’s HiSeq 2000 high-throughput sequencing instrument.LignocelluLolytic enzymes and genes involved in several ralated metabolic pathways were annotated based on the transcriptome sequence of Pseudomonas sp.E4-1.Transcriptome sequencing showed that there are 2196 assembled unigenes totally,1653 annotated KEGG. We used KO analysis for the predicted unigene, which comments to the relevant enzyme degradation of lignocelluLoses included endoglucanase,alpha-glucosidases, 1,4-beta-xylosidase,toluenedioxygenase,S-(hydroxymethyl)glutathionedehydrogenase. These enzymes which couLd metabolize benzene ring structure were likely to play a key role in the connection between the benzene ring structure in breaking lignin structure.These couLd give some help for mining-related functional genes and unctional gene cloning.