Cloning And Analysis Of DDX3Y Gene Fragment Sequences Of Male Equus Linnaeus Animals

In this study, we collected ear tissues from a healthy adult stallion (Equus caballus), a Jack donkey (Equus asinus), a male mule (Equus mulus), a male hinny (Equus hinnus) and a male foal (foal). Then we developed fibroblast cell systems and analyzed karyotypes to confirm the species. The results showed that the chromosome karyotypes of the stallion, Jack donkey, male mule, male hinny and male foal were 2n=64(XY),2n=62(XY),2n=63(XY),2n=63(XY),2n=62(XY) respectively. The results of karyotypes consisted with the results of previous studies.Based on the DEAD Box 3, Y-linked (DDX3Y) gene of human, mice and bovine, the partial sequences of DDX3Y gene in the stallion, Jack donkey, male mule, male hinny and male foal were cloned by the methods of comparative genomics and cloning sequence. The differences between these gene fragments in five animals were analyzed by bioinformatics software. The results showed that the length of DDX3Y gene fragments which contained the latter part of exon 5 to the former part of exon 17 in stallion, Jack donkey, male mule, male hinny and male foal were 4917bp,4912bp, 4917bp,4917bp and 4918bp, respectively, that showed 99.0% sequence similarity. However, these sequences showed 55 different base pairs which encoded 521 amino acids. The CDS regions contained 5 base pairs differences, two different amino acids and there were DEAD-like helicases superfamily (DEXDc) and helicase superfamily c-terminal domain (HELICc). In addition, the results also showed that the evolutional affinity relationship of the DDX3Y gene in the stallion, male mule, male foal and Jack donkey, male hinny. This study compared the characteristics of DNA sequences, amino acid and evolutional phylogenetic relationship for the first time in five male Equus Linnaeus animals, which provided useful information of DDX3Y gene and will help further study of the mechanism of reproductive regulation in cross-breeding animals.