Study On The Cultivation By JUNCAO Spent Mushroom Substrate, The Character Of Dictyophora Indusiata

It becomed an urgent problem of investigating common Juncao kinship and building its DNA fingerprinting in order to build Juncao germplasm garden, to perfect Juncao germplasm information, and to provide a theoretical basis for further study. However, this phenomenon of Introducing from different places has led to the confusion of naming. In this study, there were some main contents that common Juncao,24 kinds of plant in Pennisetum, were studyed in genetic diversity and Pennisetum purpureum and Pennisetum spp. were conducted a reseach on cell karyotype analysis using traditional compression method. A total of 13 polymorphic primers were screened, optimization of ISSR experimental system was completed, and ISSR reaction system in P. purpureum was successfully established.1) Optimize reaction conditions for karyotyping in P. purpureumThis study which carried out using traditional squash chromosome analysis methods were processed drawn parts, pretreatment, dissociation, and the best conditions were selected. The results show that it is for optimal results of apical as materials, pretreatment method of 0.05% colchicine 2 h,1 M HCl dissociation dealing with 8 min.2) karyotyping analysis of P. purpureum and Pennisetum spp.The chromosome number of P. purpureum schumach was the same as Pennisetum spp.. The results showed that the karyotype morphology of materials were different, consisted of metacentric chromosomes and sub-metacentric chromosomes mostly. In this study, the karyotype formulas of P. purpureum schumach and Pennisetum spp. were respectively 2n= 4x= 28=20m+8sm,2n= 4x= 28= 16m+8sm+4st. The relative length of chromosome composition two plants were 2n= 4x= 28= 6L+6M2+10M1+6S,2n= 4x= 28= 4L+8M2+10M1+6S. The asymmetry coefficients of two plants were close to 50%, which demonstrated basic symmetry.3) Single factor experiment to establishe ISSR-PCR primers UBC847 reaction system and procedures in P. purpureum and to optimize the otherprimer annealing temperaturePrimer UBC847 reaction system:20 μL of the reaction system, the concentration of the primers 0.5 μM, DNA amount of 40 ng. Response program:95 ℃ denaturation for 2 min,95 ℃ denaturation 45 s,50.4 ℃ annealing 45 s,72 ℃ extension 60 s, 35 cycles,72 ℃ extension of 5 min,4 ℃ save. Other 77 primer annealing temperature were optimizing, maintaining the reaction system and procedures, only changing the set annealing temperature range.4) Cluster analysis of ISSR markersUsing ISSR molecular marker analysis, Pennisetum genetic relationships was studyed among 24 species. The results were that 13 primers screened from 78 primers amplified 303 bands, of which 297 were polymorphic bands, and polymorphic sites reached 98.0%. Experimental results show that among these 24 species have higher genetic diversity. As a result, Utilizing UPGMA cluster analysis, when the similarity coefficient of 0.826 they can be divided into four categories.5) Describle the genetic relationship of P. purpureum and P. giganteum z.x.linThey were basically similar in chromosome morphology, but there were some differences in karyotype formulas and relative length of chromosome composition, the degree of evolution of P. purpureum higher than that of Pennisetum spp.. It indicated the kinship of P. purpureum and Pennisetum spp. ISSR analysis of the similarity coefficient was 0.815 and divided into the same categories. The results of karyotype analysis and ISSR analysis were more consistent, both have a certain kinship.