The Effect Of DCC(Deleted In Colorectal Cancer)and Its Ligand Netrin-1 On The Expression Of N-cadherin

The cerebral cortex is a complex structure, composed of hundreds of millions of cells, these cells are neurons and glial fibrillary. Neuronal migration is one of the most important events in the process. Recently reports suggest that N-cadherin control neurons attached to the radiation glial cells which mediates adhesion, further promote the neuronal migration.DCC(Deleted in Colorectal Cancer) is a member of the immunoglobulin superfamily, highly expressed in the central nervous system, as the Netrin-1 receptors are induced axon guidance, casting and other functions. A recent study show that DCC promote cell adhesion function.We hypothesized that DCC may affect the production of N-cadherin,then affect the adhesion between cells. In this experiment,we construct different DCC mutations :p3×Flag-CMV-DCC-ECD and p3×Flag-CMV-DCC-ICD. Then overexpress the mutations.We overexpressed p3×Flag-CMV-DCC-ICD and transfected psuper-DCC-sh RNA,we found the expression of N-cadherin and β –catenin has been reduced. Netrin-1 stimulation can not remedy the down-regulation of overexpression of DCC-ICD. And we found the transfection of psuper-DCC-sh RNA reduced the RNA level of N-cadherin. Therefore, our results suggest that DCC could affect the adhesion between cells through N-cadherin, thereby affecting the neural development.