Study Of Aminated Nano-TiO₂ Synthesis And Immobilized Lipase

With the development of nanotechnology, genetic engineering and proteomics, it was found that nano-TiO2 has a good adsorption and biological affinity, and the surface contains a large number of hydroxyl groups, making it easily to interact with other active groups, thereby producing nano-TiO2 with contain a variety of functional groups of surface that can be better used in enzyme immobilization, separation and purification for protein. Material modification of the amino function is a common modification means, has been reflected in a good adsorption properties and applications in the immobilization of protein (enzyme). In recent years, nano-TiO2 amino modification is gradually to be known. However, the existing methods for preparing amino modification process is difficult, and the amino group content is low, therefore, seek a simple and efficient method for preparing the preparation of the carrier is important research goal.The effects of experimental conditions on amino nano-TiO2 prepared in situ modification were studied in this paper which used tetrabutyl titan ate as Ti source, and lysine as the modification agent. Then the materials were characterized by Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscope(SEM) and Thermogravimetric Analysis (TG). Results showed that the nano-TiO2 were mostly about 10 nm in thickness arranged scales densely and 2.8 mmol-g-1 in amino content under the conditions of 4.0g lysine, pH 10.0 and 100℃. Consequently, amino nano-TiO2 were successfully prepared by the in situ modification of lysine, which could also the amino control content and morphology structure on the nano-TiO2 surface.The amino nano-TiO2 was prepared by modification in situ and the thermal stability through calcinations was analysed at different temperaturs. The materials were calcined at different temperatures then characterized by Fourier Transform Infrared Spectroscopy (FT-IR) and Scanning Electron Microscope(SEM).Thermal stability was investigated through DTA-TG. Consequently, calcination below 500℃ has no impact on the morphology and structure of materials, amino groups still exist; after calcination at 300 ℃, its stability has also improved.We used the amino nano-TiO2 as a carrier immobilized lipase basis on this material had synthesized in-situ modification early. Bying FT-IR、 SEM、XRD、TG and the specific surface area analyzer study of the interface characteristics of the immobilized lipase.With lysine in situ synthesis method of the amino nano-TiO2 as adsorbent, explored the binding reaction、the material transport and energy exchange between the amino nano-TiO2 and lipase. Through build up the adsorption model, calculated kinetics and thermodynamics parameters to study the adsorption process between amino nano-TiO2 lipase.