The Research Of Gene Expression Differences And Function On Nih3t3 Cell Cycle From Transcriptome Level

In order to study the gene expression differences and function of NIH3T3 cell cycle at the transcriptome level, the NIH3T3 cell cycle models were established by serum deprivation/replenishment, and the expression changes of genes were detected using Mouse Genome 430 2.0 array,and the regulatory roles of these genes in cell cycle progression were analyzed by using IPA software, QIAGEN and KEGG PATHWAY. Then explore the effection of c-FOS and NKX2-5 on the cell cycle of NIH3T3 by lentiviral vectors. The results show that the expressions of 891 cell cycle genes and 4659 genes associated with other physiological activity changed remarkably during the cell cycle. 4659 genes participated in other physiological activity with 2349 genes up-regulated, 2255 genes down-regulated, and also 55 genes up/down-regulated; 891 genes participated in NIH3T3 which regulated the cell cycle checkpoint with 548 genes up-regulated and 336 genes down-regulated. 114 genes that participated in cell cycle signaling pathways have obvious changed. Furthermore, the gene synergy was analyzed by spectral function Ep(t) based on their expression profiles. The results revealed that the activity of signaling pathways about PI3 K, STAT3, Calpain, Rho and VEGF were enhanced at G0/G1, G1/S, S, G2/M and M-phase in NIH3T3 cell cycle, respectively. Correspondingly, cell cycle regulated were also significantly enhanced. Our records suggest that PI3 K, STAT3, Calpain, Rho and VEGF signaling pathways mediated G0/G1, G1/S, S-phase, G2/M and M-phase transition in NIH3T3 cell cycle. The c–FOS and NKX2-5 were transfected through the lentivirus transfection after data analysis. The first comprehensive research about the signaling transduction of the NIH3T3 cell cycle in this paper. To research the effection of c-FOS and NKX2-5 in NIH3T3, we found that only c-FOS can express excessively. The NKX2-5 expression in NIH3T3 is very low. Further conclusion after consulting relevant references, c-FOS not the c-FOS transcription factor can induce unlimited proliferation; When NIH3T3 cell differentiate into the pluripotent stem cell, NKX2-5 can excessively express in them and cause the pluripotent stem cells to transform directionally myocardial cells.