Precursors Conversion And Purification Technology Of Coenzyme Q₁₀ Production By Bacterium

Coenzyme Q10(CoQ10) is a fat-soluble benzoquinone widespread in the nature. It is involved in energy production and activation in the human body cells. And it’s also the most efficient preventable antioxidant of arteriosclerosis. This study was designed to perfect an efficient high-yield production of CoQ10and purification technology.The effect of medium, cultivation conditions and feeding strategies on the highcell density cultivation of Sphingomonas sp. ZUTE03was evaluated. The15.71g/L concentration of cell density was obtained under the optimized parameters finally. Then, CoQ10production by alginate gel-entrapped high-density-cells of Sphingomonas sp. ZUTE03in three-phase fluidized bed reactor was conducted. The yield reached468.71mg/LThe capacity of CoQ10production and the optimal conversion conditions of Agrobacterium tumefaciens AGL1were evaluated. The optimum conversion conditions were as follows:50mg/L PHB and80mg/L solanesol as the precursors,30℃,50mL two phase system (25mL hexane as organic phase and25mL PBS (pH6.8) as water phase),2%soybean oil as a cell membrane permeability accelerant. After9h of conversion under the optimal condition, the volume yield and the specific yield of CoQ10could reach96.4mg/L and118.7mg/g respectively.Then, the condition of CoQ10purification by macroporous resins to separate was evaluated. HPD300was determined as the suitable materials for separation after adsorption test. The optimum conditions were as follows:The adsorbent and desorption solution were methanol and70%ethanol respectively. The loading amount of sample into the resins column was6BV (bed volume) at a loading rate of1.5mL/min. Then it was eluted for3h with70%ethanol at the rate of1.5mL/min, and for2.5h with acetone. The purity of the raw CoQ10increased from56.3%to96%with a recovery of69%. After silica gel purified, the purity of the CoQ10sample increased to99%with a recovery of91%.