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Biological Activity Research On The Extracts From The Spirulina Platensis Cultivated In Yunnan

Posted on:2015-08-11Degree:MasterType:Thesis
Country:ChinaCandidate:F J DengFull Text:PDF
GTID:2180330467485432Subject:Pharmaceutical engineering
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Spirulina is a photosynthetic, filamentous, helical-shaped, multicellular and green-blue microalgae. The two most important species of which are Spirulina platensis and Spirulina Maxima. Its chemical composition includes proteins, carbohydrates, essential fatty acids, vitamins, minerals and pigments like carotenes, chlorophyll a and phycocyanin. Spirulina is considered as an excellent food, with corrective properties against viral attacks, anemia, tumor growth and malnutrition.In this paper, the spirulina platensis cultivated in Yunnan was taken as the research object. Firstly, different polar solvents were used for hierarchical extacting.The four fractions of petroleum ether, chloroform, ether and methanol were gotten. The phycobiliprotein was extracted from the spirulina platensis by the method of re-freeze-thaw.The purity of C-phycocyanin obtained after chitosan affinity precipitation was0.79. The polysaccharide(PSP-1) was obtained by the method of hot water extraction and cetyl trimethyl ammonium bromide precipitation, the content of PSP-1measured by pheno-sulfuric acid method was89.2%. Secondly, as guided by cytotoxic activity, the PSP-1showed potent cytotoxicity against HepG2cells in vitro by the method of MTT, IC50value of16.9±0.6μg/mL. Finally, the polysaccharide(PSP-2) was obtained by the method which improved using trichloroacetic acid (TCA) precipitation of proteins,10000Da ultrafiltration membrane retention. The content of PSP-2measured by pheno-sulfuric acid method was97.3%. Infrared spectroscopy and elemental analysis preliminary showed that, PSP-2is a sulfated polysaccharide pyranoid. Gas chromatography showed that PSP-2contains rhamnose, arabinose, xylose unit. In vitro activity tests showed that the PSP-2on the activity of HepG2cells are better than the PSP-1, IC50value of2.6±0.2μg/mL.
Keywords/Search Tags:Spirulina platensis, C-Phycocyanin, polysaccharide, Isolation andpurification, Anticancer activity
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