Study On Expression Of Ginsenoside-α-rhamnosidase Gene

In this paper, gene ginsenoside-a-rhamnosidase was expressed in E.coli BL21,and subcloned into expression vector pPIC9K, then transformed into Pichia pastoris GS115.Containing gene ginsenoside-a-rhamnosidase, E.coli BL21was cultivated overnight in37℃,150rpm and re-inoculated into fresh TB medium, then added IPTG for induction in logarithmic phase. After separating the expression products by breaking the cell wall, the products were detected by TLC and SDS-PAGE.And Gene ginsenoside-a-rhamnosidase was subcloned into an expression vector pPIC9K, the Pichia pastoris GS115cells were transformed with the recombinant plasmid by electroporation and expressed.The results showed that, in E. coli expression, the expressed gene was no activity, and in Pichia pastoris expression, the protein not only has the activity, its molecular weight determination was also in line with the theoretical value.