The Design, Processing And Application Research Of A Device Used To Study The Culture Cell Tolerance Of Hydrodynamic Shear Force

The microcarrier culture is the most popular method used in the industrial scale cell culture. More and more researches have proved that the hydrodynamic force would damgae the cell. It may reduce the cell vitality and the adhesive ability. But the relevant studies is lack and the experiment method is also inadequate.In this research, a new type of micro-fluid device was designed to study the effect of hydrodynamic force on animal cells culture employing the computational fluid mechanics. This micro-fluid device could generate an evenly and constant distribution of hydrodynamic force. And the values of the force had a close relationship with the rate of liquid flow.Two kinds of the micro flow devices were designed for cell suspension culture and microcarrier culture. The simulation result was verified by PIV technology for the complex one. The flow field getting from simulation was very similar to the result obtained by PIV. This kind of very good anastomotic indicated that the models and calculation results are accurate.The shear tolerance of MDCK and PK cultrued on micro-carriers was tested by this designed micro-fluid device. The result showed that there was a critical energy dissipation rate (EDR). If the EDR was higher than this value, the hydrodynamic force would lead the cells injured. For microcarrier culture, the critical value was between103W/m3and104W/m3for cells cultured on micro-carriers. At the same time, it could be found that the PK cells endurance capacity of hydrodynamic force (critical EDR was5000W/m3) was better than MDCK cells(critical EDR was3000W/m3).