Preliminary Study On The Function Of The VQ Motif Protein Gene AtARVQ1in Arabidopsis Thaliana

VQ motif protein, a highly conserved protein family in plant, can interact with the transcription factor WRKY, and plays an important role in plant growth, seed development and stress response. AtARVQ1is one of the34members of the VQ motif protein gene family in Arabidopsis and participate in the Arabidopsis response arsenate stress process. In order to investigate whether AtARVQ1is involved in other stress responses and its mechanism, this study constructed the overexpression vector of constitutive promoter CaMV35S drived AtARVQ1, used Agrobacterium tumefaciens mediated transformation of wild type Arabidopsis. Seed germination rate was determined in AtARVQ1overexpression transgenic material and T-DNA insertion AtARVQl material (vql2-1and vq12-2) after treated with NaCl, ABA and mannitol, and root length was determined in AtARVQ1overexpression transgenic material after treated with NaCl. We constructed expression vector of AtARVQ1and GFP fusion, and expression vector of AtARVQ1promoter (PAtARVQ1) drived GUS. Transgenic materials were obtained using these vectors transformated Arabidopsis thaliana, these transgenic materials were localizatied at subcellular level and tissue specific expression of AtARVQl gene.The main results are as follows.1. Ten overexpression lines of AtARVQ1were obtained through antibiotic selection, PCR identification, GUS and RT-PCR analysis respectively.2. The seed germination rate in transgenic Arabidopsis of overexpression AtARVQl was significantly higher than those in control under150mMNaCl. All Arabidopsis seeds can germinate under100mM NaCl, but Arabidopsis seeds can not germinate under200mM NaCl.3. The root length in transgenic Arabidopsis seedlings of overexpression AtARVQ1was longer than those in control under100mM NaCl, but Arabidopsis seedling roots can not growth under150mM and200mM NaCl.4. The seed germination rate in transgenic Arabidopsis of overexpression AtARVQ1was slightly higher than those in control under0.5μM ABA or200mM mannitol. Seeds can not germinate under1.5μM ABA or300mM mannitol.5. The seed germination rate has no significant difference between vq12-1and wild type. The seed germination rate in vq12-2was significantly higher than those in wild type under150mM NaCl、0.5μM ABA or200mM mannitol.6. There was a strong green fluorescence signal in35S::AtARVQl-GFP and PAtARVQ1::AtARVQl-GFP transgenic Arabidopsis roots with confocal laser scanning microscopy. This indicated that the fusion proteins expressed.