The Mechanism Of Lipid Degradation Under The Involvement Of Autophasv In Livomvces Starkevi

Autophagy is conservative in eukaryote cells. Of course, autophagy will form a two-membrane organelle called autophagosome which wrap many kinds of substrate such as protein or organelles and are conveyed to vacuole or lysosome for degrading and recycling. Autophagy plays an important role in cell, such as extending life span. Researchers have found that Autophagy can degradate lipid in mouse liver cells.Lipomyces starkeyi is a oleaginous yeast in industry. It can use a variety of carbon source and can be effectively accumulated lipid as high as70%. More and more people begin to pay attention to it.Based on the genome data of L. starkeyi, we identify15autophagy core genes and analyze the interaction among key autophagy-related proteins in L. starkeyi. The analysis of the expression of autophagy conjugated system implys that autophagy is negatively correlated with lipid accumulation in L. starkeyi. Autophagy accelerator-rapamycin can reduce lipid accumulation in L. starkeyi which indicates autophagy can degrade lipid. Transmission electron microscopy images of L.starkeyi show that autophagosome is located in lipid droplets. These results confirm that autophagy is involved in the degradation of lipid in L. starkeyi.Currently, lipophagy is only be found in the mouse liver cells and fat cells. Therefore,our research not only expand the understanding of lipidautophagy but also provide a new way to improve the lipid yield in L. starkeyi.