| Tissue engineering in recent years scientific research topic in the field of life sciences an d engineering. Theory of life science, tissue engineering principles and techniques of engineering s cience combination. Formed to repair and improve the damaged tissue to replace damaged organs. Construction principle study theory to clinical application, paid more and more attention. It is prim arily functional in vitro cells through the formation of biomaterials and tissue structure and functio n of complex, surgically implanted into the body to play a role in treatment. But iPS cells is anot her fast-growing tissue engineering research. This paper has good biocompatibility and excellent pe rformance of polyethylene glycol diacrylate(PEGDA) Hydrogel biomaterials research. Hydrogel mat erial films by light, two light functional processing of Hydrogel materials.PEGDA hydrogels on fib roblasts was studied, EC109 cell, cardiac muscle cells, nerve cells and iPS cells in culture. Control led by patterned on cell growth; its influence cell growth and metabolism by MTT method evaluat ion activities obtained PEGDA Hydrogel for cell growth. Engineering research provides theoretical support for the organization. The thesis work are:(1) based on polyethylene glycol diacrylate(PEGDA) as raw materials, preparation of vis coelasticity of biomaterials, Hydrogel for bio-films. 365 nm UV nitrogen protection light 5 minutes on the polymerization of PEGDA Hydrogel for a light, excellent prepared with visco-elastic and ca n control the Hydrogel Functionalized materials.(2) with had repeatedly experiment compared using visible and the Iraq red y as raised a gent respectively in inverted microscope and laser total focused microscope Xia with 515 nm laser in suitable illumination Xia on water gel glue material for II times can controlled light aggregate r eceived branch RGDS adhesion peptide modified sex, respectively for inverted microscope 10 seco nds-5 minutes, laser total focused microscope Xia scan 4 times each each graphics maintained time for 100 ms preparation out function of of water gel glue material. With regionalization of grafting and growth of patterned features.(3) Functionalization of Hydrogel materials with cell adhesion through the MTT test PEGDA Hydrogel on the functional growth of cells and cell lines. Modified Hydrogel materials is prov ed suitable for cell growth.(4) The isolation and purification of mouse fibroblast cells and mouse fibroblast cells wer e cultured; passage and vitrified Cryopreservation of fibroblast; then the fibroblasts were recovering.And Hydrogel on the MEF was MTT test. Results showed that fibroblasts purity is high, vigorou s growth, treatment, grows well in the Hydrogel.(5) The isolation purification of myocardial cells in mice and on myocardial cells in m ice was training, as well as on mouse cardiomyocyte beating was observed. For biological material surface on cell adhesion and providing cell growth experiment as control group. Results showed t hat the high purity of myocardial cells in mice, beating lasting strong, can be used as seed cells of tissue engineering research.Primary isolation and purification of mouse nerve cells of small rat neurons were cultured in the West, and nerve cells are also routine staining and immunofluorescen ce staining. For further study of Hydrogel Functionalized growth experiments on cell adhesion after providing a source of cells. Results showed that neurons in mice a high purity, and staining char acteristics. Can be used as seed cells of tissue engineering research.(6) the development of iPS cells, and culture iPS cells was purified by flow cytometry,preparing embryoid bodies, proved that i PS activity and versatility, explores the iPS in the Hydro gel on the growth characteristics. Results showed that iPS can gel surface water. |
PDF Full Text Request