Preliminary Study Of Roles Of Epigenetic Factors In Drosophila Primordial Germ Cell Proliferation And Differentiation

The proliferation and differentiation of germline stem cells are regulated by multiple signaling. During the development of Drosophila goand, primordial germ cells’(PGCs) proliferation and differentiation are regulated by both intercellular and extracelluar signaling, due to them, PGCs could proliferate and differentiate properly. In fact, not only the wellknown signaling have participated in this process but also epigenetic factors. Scince 2005, ISWI was found as an important factor in germline stem cells(GSCs) maintance, epigenetic factors are regard as an important part in GSC regulation. Our team focuse our attention on studying epigenetic factors in PGC and GCS since 2010, and we’ve found that dSet1,dBre1,Enok,Brm ect play an important role in regulating GSC maintance, but we know little about whether those epigenetic factors could participate in regulating the proliferation and differentiation of PGCs.dBre1 is an E3 ubiquitin ligase and dSet1 is a H3K4 trimethylationase in Drosophila. In this article we’ve found that dSet1 could regulate the differentiation of PGCs depend on BMP signaling, in dSet1 knocking down PGCs, bam start transcript in an early time and the expression of pMad is decreased, what’s more even fusome exsited. And both dSet1 and dBre1 play a role in regulating cell interaction between PGCs and ICs, when dSet and dBre1 was knocking down in IC, not only the number of IC has decressed but also the number of PGC has increased.In this article we’ve found some evidence that epgnetic factors may play a role in regulating the proliferation and differentiation of PGC, and those evidence may inspire those who will have a futher study on epgnetic factors.