Cloning And Analysis Of Gene OsLZTF In Rice

Drought stress is an important factor in affecting plants growth and development. Plants are likely to be infringed by drought stress throughout the growing period. Drought stress will reduce seed germination, suppressing root growth, reducing plant height, reducing leaves, inhibiting photosynthesis, delaying heading and flowering, reducing yield and seed quality. But at different growth stages, the sensitivity of plants to drought stress is different. Plant as a whole, all the tissues have undergone a series of physiological and biochemical changes when drought stress occurs.Rice like high-temperature and high-humidity. Drought stress will not only reducing production, but also seriously affecting the quality of grain. With the increasing shortage of freshwater resources around the world, how to improve rice’s drought resistance has become an important direction of biological science. With the rapidly developing of molecular biology, the study of plant’s drought resistance has achieved many important achievements at the molecular level, which provides a theoretical basis for drought-resistant rice’s molecular breeding. By transgenic methods, changing the expression of drought-related genes can obtain higher drought resistance plants in relatively short time, shortening the breeding time.OsLZTF (Gramene:LOC_Os02g43330), located on rice chromosome No.2 OsLZTF’s length is 1423bp, containing two exons, one intron. The cDNA length is 1336bp. The entire coding frame length is 786bp, encoding a protein which have 261 amino acid. The protein is about 29KD. By quantitative PCR reaction, we find that the gene have different levels of expression in Nipponbare roots, stems, leaves. The expression can change with the drought stress time. So we guess that the gene is relating to drought resistance in rice. Using bioinformatics software, we designed gene-specific primers to amplify the gene from Nipponbare leaves cDNA.And we constructed overexpression and suppression vectors. Then we used Agrobacterium-mediated system to make the gene get into Nipponbare. At last we get transgenic plants and seeds.The main results are:1. We successfully cloned OsLZTF from the leaves cDNA of wild-type Nipponbare, and we conducted bioinformatics analysis about OsLZTF.2. Using quantitative PCR reactions, we analyzed OsLZTF gene expression levels in various tissues of Nipponbare. We found that without drought treatment, the gene had different levels of expression in roots, stems and leaves. And during drought treatment, the expression in various tissues can change with the time of drought stress.3. We constructed overexpression and suppression vectors, established a appropriating genetic transformation for rice with Agrobacterium-mediated manners. Then, we get transgenic plants andTO generation seeds with greenhouse cultivation and taken to Hainan for reproduction.