| Salvia przewalskii Maxim., a kind of perennial herbaceous plants, is a member of Lamiaceae, Salvia Linn. As a relatives of Salvia miltiorrhiza, Salvia przewalskii has some medicinal ingredients such as salvianolic acid, tanshinone et al. detected in the dry roots and rhizome of Salvia miltiorrhiza. And it is used as a substitute of traditional chinese medicine Danshen for circulation in the country. However, existing studies show phenolic acids levels of Salvia przewalskii is low. Therefore, the main purpose of this study is to explore the influence of elicitor on the accumulation of some phenolic acid in the seedling of Salvia przewalskii Maxim, which could provide theoretical basis for the effective use of Salvia przewalskii Maxim.. The main research results are as follows:1. Different doses salicylic acid (SA) treatments brought various effects on the physiological indices of primary and secondary metabolism of Salvia przewalskii seedlings. Overall, plant metabolism was stimulated by low concentrations SA treatment (0.5mmol, 1mmol or 3mmol). Soluble protein and soluble sugars concentrated to the highest in 0.5mmol and 3mmol SA treatment group respectivly. PAL and PPO activity increased significantly in 3mmol SA treatment group. TAT enzyme maintained a high activity under low concentrations of SA treatment. Accumulation of Phenolic compounds (rosemary acid, Sal B and Protocatechualdehyde) reached the maximum in 3mmol SA treated group. High concentration of SA treatment had inhibition effect on Salvia przewalskii seedlings metabolism.2. Effects of Ag+ on Salvia przewalskii seedlings depended on the Ag+ concentration and prolong time. Accumulation of soluble sugar and soluble protein in the seedling was promoted by Immol and 0.5mmol Ag+, and the PAL enzyme activity was stimulated to a higher level. Also the highest levels of rosemary acid, sal B and sal A were detected in Immol Ag+-treated group, the highest levels of dihydrotanshinone, tanshinone I and tanshinone IIA obtained from 0.5mmol Ag+ treatment group. High concentrations of Ag+ treatment resulted in decreased metabolism of seedlings, moreover, this inhibitory effect increased with time prolongs.3 In light-elicitation experiments, the blue and red light had a strong induced effect on PAL, TAT enzyme activity. Thus the content of soluble protein was higher in the red and blue light group than other group. Secondary metabolite content test results showed that Sal A, Sal B, dihydrotanshinone, tanshinone IIA, tanshinone I concentrated to the highest in the blue light treatment, rosemary acid and protocatechualdehyde accumulated in the red light treatment were highest. Effect of yellow light on plant metabolism was not obvious, while the green light had a certain degree of inhibition. |
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