Studies On Screening Of Phosphate Solubilizing Bacillus Megaterium And Its Industrial Application

Phosphate solubilizing Bacillus megaterium as agro microbial agent commonly used strains, with a variety of functions of phosphate solubilizing, promoting, biocontrol, etc.. Therefore, especially important to breed excellent solubilizing ability of strains, the purchase from 6 strains of phosphate solubilizing Bacillus megaterium bacteria were selected with a clear function of the strain, and the conditions of growth, the culture medium, the industrial production conditions of the process.6 strains of bacteria are able to decompose the sparingly soluble inorganic P Ca3(PO4) 2. According to the content of available phosphorus in different bacterial culture supernatant, selected from the J1, J2 J3 of three strains of Bacillus megaterium bacteria have phosphate solubilizing effect in soil phosphorus solution, which J3 has the best result, after 30 days of culture, the effective phosphorus content in soil from 20.68mg/L to 35.63mg/L. Instead of phosphate solubilizing bacteria LA from 20.68 to 21.52mg/L. Phosphate solubilizing effect is obviously better than that of non phosphate solubilizing bacteria. Preliminary determination of J3 phosphate solubilizing Bacillus megaterium bacteria as the next pot experiment with bacteria. Add 40000000 / basin J3 phosphate solubilizing Bacillus megaterium bacteria 45 natural plant long-term end statistical average dry weight, yield up to 19.6%, the increase of the maximum, to determine the optimum adding amount of phosphate solubilizing bacteria J3. Using field plot test results analysis of variance shows: the application of agricultural microorganism solubilizing bacteria J3 obvious yield increasing effect, treatment 1’s is 3 corn yield 47.3 kg / mu, the yield increase rate of 8.08%, very significant differences. Treatment 2’s is 3 corn yield 1.3 kg / mu, increase rate is 0.23%, the difference was not significant。Through the single factor experiment, determined the J3 phosphate solubilizing Bacillus megaterium strain 250 ml triangle bottle best installed capacity is 25 ml. The optimal growth PH 7, the optimum growth temperature of 37 ℃. Determination of 2 of molasses as the carbon source, corn steep powder as nitrogen source, inorganic salt is CaCL2. By optimization of culture medium formula orthogonal experiment, that is the optimal formula for corn steep powder 2%, molasses, 5%, CaCL20.06% K2HPO40.3%. Inoculation amount 0.01% or 0.1% can meet the need of production. The optimum medium of fermentation in 10 L canister, when the ventilation rate reached 1.5, speed can reach 350r/min when the maximum number of bacillus. According to the growth curve of the preliminary determination to put pot time between 24-28 h, sampling time in 12-16 h, 20-28 h microscopy in the spore rate above 95% placed tank. 200 r/min which speed reached at 1000 L and 5000 L tank, ventilation volume reached 1.5 spore number reached the maximum. Identified as the basic liquid reaches the 1200 ml centrifugal high yield. Determination of high speed mixing time in 10 min, to determine the optimum drying temperature at 45℃, display temperature storage products best below 30℃.