Study On Surface Plasma Resonance Immune Sensing Detection Of Sulfonamides

Sulfonamides (SAs) are a group of synthetic antibiotics. They have been widely used in animal husbandry and aquatic products breeding to prevent and treat a series of diseases in bacterial infectious diseases. Excessive use of SAs can cause drug residues, pose a threat to human health and produce toxic effects, allergic, dysbacteriosis or other hazards.Based on surface plasmon resonance immune sensing technology, a rapid, efficient and accurate detection method for the detection of SAs residues in animal food was developed.This study used amino coupling way to modify the coating antigen onto the surface of a sensor chip. Then use the way of inhibition to make the solution mixed with SAs and antibody flow through the surface of the SPR sensor chip and build the four kinds of SAs (SQ、SCPA、SMT、SPMX) standard curve. In order to verify the accuracy and usefulness of the method established in this study, we constructed curves of eliminate the influence of matrix. Then add different concentration levels (10 ng mL-1,20 ng mL-1,30 ng mL-1) SAs into samples. Finally, we use the established analysis method for testing after a simple handing and extraction process.The results of four kinds of sulfa drugs (SQ、SCPA、SMT、SPMX) exhibited a lower detection limit of 3.87 ng mL-1、3.28 ng mL-1、4.92 ng mL-1、3.58 ng mL-1 and the values of IC5o were 17.66 ngmL-1、17.25 ng mL-1、19.85 ngmL-1、19.92 ng mL-1, respectively. The added concentration at each sample recoveries of four sulfonamides were between 84.81%-106.20% and the relative standard deviation (n= 3) were less than 7.1%, indicating that the established detection method has good accuracy and applicability in rapid detection of sulfonamides in food samples.