Effects Of Electric Field Treatment On Panicum Miliaceuml Seeds Germination And Dence Expression In The Young Leaves

In this study,Panicum miliaceum L seeds were used as experiment material.24 kinds of electric field were used to treat the Panicum miliaceum L seeds.Experimental determination was used to test the effects of electric field on the germination rate,and selected out the electric field condition,which improved Panicum miliaceum L seeds germination rate significantly. mRNA differential display reverse transcription PCR (DDRT-PCR) was used to select out the different expressed bands that the treatment group compared with the control group. Recycling the different expressed bands and sequencing the differential fragment to test which condition of electric treatment can influent the germination rate of the Panicum miliaceum L seeds.The results showed that the electric field E425、E435、E525、E615 and E6110 had a significant effect on Panicum miliaceum L germination rate, and the electric field E525 had a highly significant effect on Panicum miliaceum L germination rate.DDRT-PCR results showed that compared with the control group,the treatment group appeared 186 bands and disappeared 172 bands.Two different expressed cDNA fragments are obtained by sequencing and verifying. One of them is Dl which is the induced expression sequence by electric field treatment. D2 is the inhibited expression sequence by electric field treatment. Using Blastn for the sequence comparison,we found that there is no highly similar sequence to D1, and D2 is similar to Setaria italica microtubule-associated protein sequence (LOC101779666) and the similarity is 91%.It indicated that a certain electric field treat the Panicum miliaceum L seeds could improve germination rate and influenced its young leaves gene expression. This result provides the experimental basis for analyze the micromechanism of the macro-biological effects on electric treatment plants seeds.