| Root not only provides physical support for plant growth, but also absorbs water, salts and other nutrients from the soil to supply plants growth. In order to adapt to the complex environment of the underground, there are clear functional regions of plant roots. Structurally different root regions make right response to different environmental signals to perform different physiological functions by receiving-analysis-answer mode. This study investigated different growth of root regions between growth patterns and different light photoreceptional signals in the model plant Arabidopsis thaliana. We analyzed root length of different Arabidopsis mutants (phya, phyb, phot1, photlphya, photlphot1). The result indicated different photoreceptors influenced the growth and development of Arabidopsis root. Furthermore, the use of real-time PCR technology to analyze differential expression of different light receptor gene (PHOT1, PHOT2, CRY1, CRY2, PHYA, PHYB) and the growth of transport carrier gene (PIN1, PIN2, PIN3, PIN4, PIN7) in different regions of the roots and we found them to be partitioned signaling pathways involved in the influence of light is different in different roots. We discovered the expression of those genes and spatial position under the Semi -exposure bracket light conditions were totally different from the nomal light conditions. In this study, the applicability of this fluorescence probe in the Arabidopsis thaliana was carefully investigated. A wide-accepted method for quantitative visualization of microdomains, the GP-value algorithm, was also applied in living cells imaging in Arabidopsis. These works provided experimental evidences for quantitative visualization of membrane microdomains in plant cells. The membrane lipid of root has different degree of order. In addition to this, the membrane lipid of transition zone was well ordered. Those results indicated that the the degree of order of transition zone was closed with signal transduction. |
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